Effect Of Lactobacillus Delbrueckii Reduce Intestinal Oxidative Stress In Piglets

This study induced oxidative stress in piglets by intraperitoneal injection of lipopolysaccharide, and then studied the effect of dietary supplememtation with lactobacillus delbrueckii on oxidative stress in piglets.A total of thirty-six healthy 28-day-old crossbred (Landrace×Yorkshire) piglets were randomly divided into three treatment groups, including control group, LPS, and LPS+LAB groups, each treatment contained six replicates of two pigs. The control and LPS groups were fed a corn and soybean meal-based diet, and the LPS+LAB group was fed the basal diet with 0.2% lactobacillus delbrueckii. At day 29 of the trial. Piglets in the LPS and NAC groups were injected intraperitoneally with LPS at 100 μg/kg BW, whereas piglets in the control group were injected intraperitoneally with normal sterile saline at the same volume. Blood samples were collected 3 h after LPS challenge, and at 6 h post-injection of LPS, the pigs were killed to obtain the intestinal mucosa. The results showed as follows.(1) Between day 1 and 28 of the trial (before the LPS challenge period), no significant difference was noticed in growth performance between the treatment groups (P>0.05).(2) The effect of Lactobacillus delbrueckii on intestinal mucosal morphology in piglets challenged with lipopolysaccharide:Compared to control, LPS challenge reduced the villus height in the duodenu, jejunum, and ileum (P<0.05), and increased the crypt depth in jejunum and ileum mucosae (P<0.05), while crypt depth of the duodenum was insignificant (P>0.05). LPS significantly reduced the ratio of villus height to crypt depth in the duodenum, jejunum, and ileum (P<0.05). Compared to LPS group, LAB increased the villus height in the duodenum, jejunum, and ileum (P<0.05), and reduced the crypt depth in the jejunum and ileum (P<0.05). Moreover, LAB increased the ratio of villus height to crypt depth in the duodenum, jejunum, and ileum (P<0.05).(3) The effect of Lactobacillus delbrueckii on the level of immune factors in piglets challenged with lipopolysaccharide:Compared to control, LPS challenge reduced the level of IL-10 in the jejunum and ileum mucosae (P<0.05), the level of 1L-10 in colon decreased 15.57%. but no significantthe difference was insignificant (P>0.05), increased the level of IL-1β in jejunum and colon (P<0.05), increased the level of TNF-a in jejunum, ileum and colon mucosae (P<0.05). Compared to LPS group, LAB increased the level of IL-10 jejunum and ileum mucosae (P<0.05), reduced the level of IL-1β ileum mucosae (P<0.05), and reduced the level of TNF-α in jejunum, ileum and colon mucosae (P<0.05).(4) The effect of Lactobacillus delbrueckii on serum oxidation-related indicators in piglets challenged with lipopolysaccharide:Compared to control, LPS challenge decreased serum T-AOC, CAT, GSH-px, and GSH by 8.2%,3.8%,18.2%,9.7%, but no significant difference (P>0.05). In addition, LPS significantly increased serum MDA and H2O2 abundance (P<0.05). Compared to LPS group, LAB alleviated LPS induced oxidative stress evidenced by the increased serum T-AOC activity and reduced MDA and H2O2 (P<0.05).(5) The effect of Lactobacillus delbrueckii on mucosa oxidation-related indicators in piglets challenged with lipopolysaccharide:Compared to control, LPS challenge reduced the T-AOC ability and GSH levels in the jejunum and ileum (P<0.05) and increased the level of MDA in the jejunal mucosae (P<0.05). Compared to LPS group, LAB increased markedly increased the level of GSH in the jejunal mucosae (P<0.05) and redced the levels of MDA in the jejunal and ileal mucosae (P<0.05).(6) The effect of Lactobacillus delbrueckii on the mRNA abundance of tight junction proteins in the intestinal mucosa piglets challenged with lipopolysaccharide: Compared to control, LPS challenge increased the mRNA abundance of ZO-1 in the duodenum (P<0.05) but reduced the mRNA abundance of ZO-1 in the jejunum and colon mucosae (P<0.05). Meanwhile LPS reduced the mRNA abundance of Occludin in the duodenum (P<0.05) and increased the mRNA abundance of Occludin in the jejunum (P<0.05). Compared to LPS group, LAB increased the mRNA abundance of ZO-1 in the jejunum (P<0.05) and Occludin in the duodenum, jejunum, and colon mucosae(P<0.05).(7) The effect of Lactobacillus delbrueckii on the mRNA abundance of antioxidant enzymes in the intestinal mucosa challenged with lipopolysaccharide: Compared to control, LPS challenge reduced the mRNA abundance of CAT and CuZnSOD in the jejunum and colon mucosae (P<0.05), but increased the mRNA abundance of CAT and CuZnSOD in the ileum mucosae (P<0.05) and increased the mRNA abundance of GPx1 in the jejunum mucosae(P<0.05). Compared to LPS group, LAB increased the mRNA abundance of CAT and CuZnSOD in the ileum mucosae(P<0.05).Insumary, the conclusions of the present study are:(1) Lactobacillus delbrueckii can reduce the amount of oxidation products, increased antioxidant capacity, and increase the expression of genes of antioxidant enzymes, can alleviate oxidative stress.(2) Lactobacillus delbrueckii can alleviate the intestinal oxidative damage, the pathway may:reduced MDA and H2O2 content, reduced levels of inflammatory cytokines, upregulation of intestinal epithelial tight junction protein, increasing villus height, crypt depth reduce.