Transcriptome Analysis Of Pinellia Ternata (Thunb.) Breit Bulbil Based On High-Throughput Sequencing Technology

Pinellia ternata(Thunb.)Breit,araceae perennial herbaceous plants,is a traditional Chinese medicine and origin from Shennong Materia Medica,belonging to the one of Inferior Chinese medicine.Its dry tuber is an important of medicine product,and its characters are mild,spicy,poisonous.Pinellia ternata,which have an active effective of spleen,stomach and lung,can reducing phlegm and stopping vomit.There are three reproductive patterns: seed breeding,bulbil reproducing and tuber reproducing.In agricultural production,bulbil reproducing has a higher efficiency than others,and can achieve fast speed and high output.So bulbil is play a vital role in Pinellia ternata's yield.meanwhile Pinellia ternata's breed and quality are also very important.In our study,Pinellia ternata as materials,we use RNA-seq technology,to detect the differences in between Pinellia ternata bulbil and stem gene expression,obtaining genes related to the formation of some Pinellia ternata bulbils,and based on these to elucidate the molecular mechanism of Pinellia ternata bulbils trip,and that we also make a contribution to reproduction of Pinellia ternata.Meanwhile,it can also enrich the genetic information of databases,which is used by other researchers.After testing and quality assessment,the cDNA library was qualified and the result of the transcription group sequencing was good and feasibility.Two samples were sequenced by Hiseq2500 Illumina high-throughput sequencing platform and then a total of 7.1Gb raw data were obtained which 149825428 Reads Raw,after assembling by Trinity,Obtain 90175 unigenes,with the average length of 751 bp.Unigenes through the BLAST program to NR,KOG,Swiss-prot,Pfam and KEGG and other databases(<1e-5 value E),respectively,55197,46237,35616,38967 and 22961 notes.By compared all Unigenes,through BLAST,to NR,KOG,Swiss-prot,Pfam and KEGG and other databases(E <1e-5),we Obtain,55197,46237,35616,38967 and 22961 notes respectively.After further analysis,28 kinds of 17894 SSR loci and 205613 SNP loci were found in the transcriptome.By Analysizing Gene expression of tow samples,we have screened 85884 differentially expressed genes with 5869 up regulation and 9615 down regulation.According to statistical method,we have screened 1214 remarkable differentially expressed genes with 687 up regulation and 527 down regulation.GO enrichment analysis of differentially expressed genes,the results showed that there were 18766 genes enriched to the cell components,11628 genes were enriched to biological processes,and 8285 genes were enriched to molecular function.After enriching Differentially expressed genes in KEGG Pathway,a total of 30833 differentially expressed genes were enriched to 246 KEGG Pathway and 5627 significantly differentially expressed genes were enriched to 246 Pathway KEGG.By GO and Pathway enrichment analysis,5 differentially expressed genes were enriched in the pathway of ZR.The results showed that the gene expression of ZR synthesis pathway in the bulbils was higher than stem,and the gene expression of the ZR decompose in the stem was higher than bulbils.12 differentially expressed genes were enriched in the IAA biosynthesis pathway.The gene expression of the IAA biosynthesis pathway in the bulbils was higher than stem.8 differentially expressed genes were enriched in the biosynthetic of the JA.The gene expression of JA synthetic pathway in the bulbils was higher than stem.There are 3 genes that are enriched in the ABA biosynthesis,and the expression in the bulbils is larger than stem.There is a gene that is enriched in the biosynthesis of GA,the expression of this gene in the stem is larger than bulbils.