Study On The Selection Of A Bacterium Strain To Against Vibrio Parahaemolyticus

Screening of deputy hemolytic vibrio efficient bacteriostatic strains can bring great contribution for the production.The strain of Vibrio parahaemolyticus Vp1 was selected From the sick vannamei,using its screening 7 Bacillus A,B,C,D,E,F,G were selected,B,C,D,E,F,G are Bacillus licheniformis,A is a Bacillus amyloliquefaciens,which have strong inhibitory effects to Vp1.For safety test,because A and C bacterium have hemolysis phenomenon.Bacteriostatic rings off B,D,E,F,etc are smaller.So,follow-up experiments using G bacterium,for the biological characteristics experiment and the protection of deputy hemolytic vibrio infection of zebrafish effect experiment.G bacteria in 32?,pH of 5,0.5%salinity,grows the best.Acute toxicity:artificial infection by intraperitoneal injection of zebrafish 4x108CFU/mL,12 h all died,it can be used as pathogenconcentration in subsequent protection experiments.Vpl(2.046x108CFU/mL)and G bacteria(1.5375 x108CFU/mL),have the protective effect of 50%.The results showed that G strains to Vp1 has good control effect.Finally,the strain G was selected which has particularly good effects.It can be used to develop new biological agents,to bring the Gospel to the aquaculture industry.