Vitro Culture Of Glochidia And Early Juvenile Cultivation In The Cockscomb Mussel Critaria Plicata

In vitro culture is an artificial propagation method that allows mussels to undergo their metamorphosis from larvae to juveniles without the use of a host fish.This paper carried out the glochidia in vitro culture technique on the development of non parasitic metamorphosis of the larvae survival.growth and development of the track of Cristaria plicata.And carry out the early larvae culture screening and optimization model.In vitro culture is good for which lack of the host fish data freshwater mollusks,it could protection and recovery freshwater shellfish,and provide a new way for mussels' complete artificial propagation.1.Study of a suitable fish plasma for in vitro culture of glochidia Cristaria plicataThe freshwater pearl mussel,Cristaria plicata was cultured in artificial media at 24±1?.Each artificial medium contained a mixture of L-15.host fish-bighead,non host fish--yellow catfish,carp,tilapia artificial medium plasma as a protein source,and antibiotics/antimycotics at a ratio of 2:1:0.5,and set up control group.Study of a suitable fish plasma for in vitro culture glochidia Cristaria plicata;Analysis of biochemical indexes in the serum of 4 fishes;Test the effect of sediment and light on the growth and development of juvenile.The results show that:the control group of glochidia survival rate was(76.24±6.71)%and transformation rate was 0;The transformation from glochidia to juvenile stage was up to96.26?99.11%in four fish plasma,transformation rate have the difference was significant that in the no host fish group higher than of specific host fish group(P<0.05);It have significantly that high density lipoprotein is higher than low density lipoprotein in 4 kinds of serum;The survival in the sediment and light was 93.33±0.27%,and the shell length of survival juvenile was 1.06±0.13 mm.The survival in sediment without normal light was 92.22±0.58%.And the shell length of survival juvenile was 1.03±0.17 mm.Do not add sediment group were all died.Results showed that light had no effect on the larvae growth;sediments have a decisive effect on the larvae growth.2.Study on the formula and condition of glochidium Cristaria plicata in vitro cultureExperimental utilization carp artificial medium plasma as a protein source,Compared the basic culture fluid and serum ratio,concentration of antibiotic and training effect of density on larval metamorphosis rate and juveniles during the late growth stage.The results show that:there had no significant difference when the basic culture fluid and serum ratio2:1,2.5:0.5 and 2:0.5 cases(P>0.05).There were no significant differences in the density 300-1100(P>0.05),and the transformation rate is higher than 96%.Without the addition of antibiotics concentration group was the lowest for metamorphosis 76.24±9.47%,There were no significant differences in other groups.3.The glochidia and juvenile Cristaria plicata growth development and morphology observation in vitro cultureWe observed the morphology of glochidium and juveniles by using optical microscope and scanning electron microscope.We found that there was no significant change before metamorphosis to juvenile of shell surface.After this metamorphosis,there was difference between new shell and original shell.These signs were found to consist mainly of the mantle edge and the foot formation.Shell growth as well as the appearance of gills and the accumulation of some organic matter inside the gastrointestinal tract was also observed.The cilia of the mantle were observed from third to six days,The gill were observed from six days,The juveniles were fed for 1 months,we observed the formation gill by microscope.