Study Of Key Techniques On The Introduction Of Camellia Sinensis. Jinxuan Seedling

In order to improve the survival rate of cloning tea seedling which introduced from the southern tea area to Shandong tea region,temporary planting and shading experiments of one-year-old cutting seedling of C.sinensis cv.Jinxuan were made.Three different substrates of A1(sand:soil =1:2),A2(sand:soil =1:1)and A3(sand:plant ash:vermiculite =1:1:1),four planting densities of D1(1 plant/ cluster),D2(25 plant/cluster),D3(50 plant/cluster),CK(100 plant/cluster)and two growth regulators ABT-3(C1),GGR-6(C2)were used in the temporary planting experiments,three shading rates(25%,50%,75%)were investigated on the tea plant in late May.By comparing the physicochemical factors of the three substrates and determining the root architecture,root activity,length of young shoots,chlorophyll fluorescence parameters,antioxidant enzyme activity and survival rate,this study explored the suitable substrate,density and shading rate of tea temporary planting,researched the application effect of two growth regulators for the weather condition of Shandong province,The result is as follows:(1)A3 had the lowest bulk density and EC,the largest total porosity and water retaining porosity,the highest contents of organic substrate,available N,P,and K,and had the best warmth retaining effect.A1 achieved good effect on buffering temperature change as its diurnal temperature range was the least;Compared to A1 and A2,the new root of A3 generated earlier,the activity,length,surface area,volume,and numbers were significantly higher under temporary planting of A3;The chlorophyll fluorescence parameters(Fv/Fo,Fv/Fm,?PS?,qP)of tea seedling by A3 were higher than that by A1 and A2,but no significant difference between A3 and A2;The survival rate of tea seedling after temporary planting under three substrates were above 95%,among that by A3 was highest and amounted to 98.7%;The correlation analysis showed that the length of new root and young shoots had significantly positive correlation with the content of organic matter and available content of N,P,and K,significantly negative correlation with bulk density.Therefore,the substrate was more suitable for temporary planting which the bulk density was lower and levels of the organic matter,nutrient element content were higher.In this study,A3 had the best effect on the temporary planting tea seedling of C.sinensis cv.Jinxuan.(2)The root zone temperature of four treatments were all increased with the increasing of planting density,and they were significantly different other treatment during the dormancy period,while D1 was significantly lower than CK during the period of germination;The activity,length,surface area,volume,and numbers of new root were all showed D3>D2>CK>D1,but there was no significant difference between D3 and D2.In addition,the new roots of D3 generated earlier and the root activity was significantly higher than other treatments during the period of dormancy,D2 had the highest root activity during the period of germination,but there was no difference between D1 and D3;D2 had the highest survival rate and the longest length of young shoots,The chlorophyll fluorescence parameters(Fv/Fo,Fv/Fm,and qP)of tea seedling showed that D3>CK>D2>D1 during the period of dormancy,and it exhibited D2>D3>D1>CK during the period of germination,it exhibited D2>D3>D1>CK during the period of germination,After comprehensive analysis and comparison D2 achieved good effect on keeping temperature change and developing root,while it suffered less stress of light,moisture and nutrition,therefore it could be used as the suitable density for the tea seedling under temporary planting.(3)C2 had the highest rate of Fv/Fmand Fv/Fo during the period of dormancy and germination.The chlorophyll fluorescence parameters qP of tea seedling showed that C1>C2>CK during the period of dormancy,and it exhibited C2>C1>CK during the period of germination,while there were no significantly different in the three treatments.The activity of SOD registered as CK>C1>C2;POD and CAT activity assumed CK>C2>C1.After comprehensive analysis and comparison,C2 achieved better effect than C1 on the growth and physiological activity of tea seedlings.However,there was no significant difference between the tea seedling under temporary planting which processed by growth regulators and CK.The reason may be that the tea seedling was in the dormant period when the growth regulator used,and the low efficiency of the growth regulator could affect the development.(4)A suitable shading on the new planting tea seedlings by using the shading net technique could decreased the light intensity,air temperature,and surface temperature,and leaf temperature.While air humidity in tea garden was increased significantly,the contents of chlorophyll increased with the greater shading degree,resulted in the micro environment and physiological state of tea seedlings improved effectively.? PS? increased with the shading rate at T1(25% shading rate)and T2(50% shading rate),but decreased at T3(75% shading rate).Compared with CK,the survival rates of T1,T2 and T3 were increased by 21%,35.8%,and 33.1% after one month of shading treatment.These results indicated that 50% shading treatment could be suitable for improving survival rates and biochemical quality of new planting tea seedlings in Taian region.