| The Growth of Crassulaceae Succulents is slowly,and the cost is high because of peat substrates from other country.In order to reduce the cost and promote the growth of Crassulaceae Succulents,this study had screened substrates which can replace peat as Echeveria laui substitution from the 8 organic wastes;this study had screened plant growth promoting rhizobacteria?PGPR?from rhizosphere substrate of Echeveria laui;we study on peat substitution matrix as carriers for different PGPR of Echeveria laui;the selected organic waste and the PGPR were complex formulation,Conduct experiment of Echeveria laui.The main results are as follows:The pH of sheep manure,pleurotus eryngii residue,washed cow dung was in the proper range of substrate.The pH of donkey manure,golden mushroom residue,vermicompost was slightly beyond the scope of the proper substrate.EC of vermicompost,Peat in the plant culture substrate safety range(0.1-0.8 ms·cm-1);donkey manure,golden mushroom residue,oyster mushroom residue,washed cow dung EC beyond the scope of the substrate safety range(2.6 ms·cm-1).Several organic waste bulk density in the range of 0.1-0.8 g.cm-3.The total porosity of different organic wastes ranges from 48.25%-60.65% and the water content ranges from 3.81% to 29.07%,the water content of pleurotus ostreatus residue was highest and the water content of vermicompost was lowest.There are significant difference of contents of available N,available P,available K and organic matter in the different organic wastes.With the proportion of water extract of donkey dung,golden mushroom residue,vermicompost,peat decreased the lfalfa germination index with the proportion of water extract increased.8 kinds of organic wastes were mixed with perlite used as the substrate of Echeveria laui.The content of Echeveria laui proline was 8.36-19.88 ug·g-1 and the soluble sugar content was 0.39-0.65 mg·g-1.Cultivated with organic wastes mixed with perlite,such as donkey manure,pleurotus ostreatus residue,washed cow dung,cow dung,vermicompost,the total chlorophyll content of Echeveria laui was higher than that of peat mixed with perlite.Only vermicompost mixed with perlite as rhe substrate Echeveria laui leaf number,the crown,fresh weight,underground fresh weight that were significantly higher than that of peat mixed with perlite.By the experiment we selected four plant growth promoting rhizobacteria?PGPR?,namely Bacillus aryabhattai,Cellulosimicrobium cellulans,Micrococcus luteus,Pseudomonas aeruginosa on Echeveria laui.The present study test biomass,root morphology,chlorophyll,endogenous hormones content of E.laui.The results showed that chlorophyll content of E.laui inoculated with PGPR increase 14.77%-52.27% than control,respectively.Chlorophyll content of E.laui inoculated with B.aryabhattai was highest.Zeatin,indoleacetic acid content of E.laui inoculated with PGPR is higher than control,and Zeatin,indoleacetic acid content of E.laui inoculated with B.flexus was highest,with 9.99,11.32 times of control.Inoculating with B.aryabhattai?B.flexus?M.luteus can improve volume,fresh weight of E.laui,and volume of E.laui increase 14.09%,21.82%,27.73%,fresh weight of E.laui increase 45.83%,50.00%,41.67% than control,respectively.PGPR inprove E.laui growth,fresh weight and crown of E.laui inoculated with B.aryabhattai are highest,compared with control,fresh weight and crown of E.laui increase 38.18%,10.97%.Secondly,fresh weight and crown of E.laui inoculated with M.luteus increase 24.28%,7.83%.Plant growth promoting of B.Aryabhattai on E.laui was most significantly among 4 PGPR,B.Aryabhattai can be as a preferential PGPR of E.laui.PGPR strains suitable for adsorption carriers shows that,The release rate of B.Aryabhattai in golden mushroom residue is 104.85% and B.flexus in vermicompost release rate is 133.61%.B.Aryabhattai in golden mushroom residue and vermicompost of living bacterium within the scope of the higher number,the number of living bacterium was significantly higher than that of peat.The number of B.Flexus living bacterium in golden mushroom residue,vermicompost,peat was ignificantly lower than that of B.Aryabhattai.Golden mushroom residue and vermicompost can be used as E.laui substrate because Alfalfa germination index of 10%,25%,50% volume fraction water extract of golden mushroom residue and vermicompost have adsorbed B.Aryabhattai is higher than 80%.Using golden mushroom residue and vermicompost in different proportion distribution as E.laui substrate that is benefit of E.laui growth and the promoting effect is stronger than vermicompost mixed with perlite.The compond substrate increase the chlorophyll a and chlorophyll b,total chlorophyll of E.laui,the root biomass,surface area,volume and root length was higher than E.Laui in organic wastes mixed with perlite,such as golden mushroom residue,vermicompost,peat.The compound substrate?vermicompost: golden mushroom residue: perlite =9: 3: 8?on the growth of E.laui was the most significant,and the crown,leaf number and biomass were increased by 45.16%,21.75%,38.31% respectively compared with peat,chlorophyll a and chlorophyll b,total carotenoids,chlorophyll were increased by 24.76%,24.95%,31.96%,25.67%.B.Aryabhattai adsorbed in the substrate can promote the growth of the E.laui.The compound substrate?vermicompost: golden mushroom residue: perlite =9: 3: 8?adsorption bacteria liquid can make E.laui crown,leaf number and biomass increased by 64.58%,33.69% and 33.69%,respectively.Compared with peat,the 3th kind of substrate adsorption B.Aryabhattai can make the E.laui plant chlorophyll a and chlorophyll b,total carotenoids,chlorophyll increased by 39.15%,39.09%,47.31% and 39.09%,respectively. |
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