Transcriptome Study Of The Fruits Of A Transgeneic Strain Of Cucumis Melo L.CV Hetao Transformed With An Antisence ACO Gene During Fruit Ripening Process

Melon is a widely-planted fruit crop with great economic value.The study of biological mechanisms and related genes associated with ripening process of melon fruit is of great significance for developing a clear understanding of the fruit ripening process of melon and other plant species.Furthermore,this study will provide theoretical and practical evidence for further studies of fruit preservation and quality improvement.Bioinformatic and transcriptomic methods were used in this research to conduct RNA-seq and comparison experiment between transcriptomes of M9,a strain of Cucumis melo L.with transgeneic antisense ACO gene,and the wild type of Cucumis melo L.cv Hetao,based on the different periods of fruit development.This research aims to study the influences of antisense ACO gene on the ripening process as well as the relationship between ethylene and development of melon fruits.We performed a genome-wide bioinformatic identification of 5 gene families important for the transcriptional regulation of ethylene biosynthesis and ethylene signaling pathway in melon,including CmCTR,CmEIN2,CmHB,CmETR and CmRIN,of which 20,6,17,3 and 21 gene members were identified respectively,and together with gene family CmEIN3/EILs we studied their transcriptome data,focusing on the fruit ripening process.The results showed that most of the gene families presented complicated expression patterns.However,CmEIN3/EILs family members showed an overall up regulation during this process,while CmETR showed downregulation.We also conducted a statistical analysis of the GO data within the transcriptomes of both strains,summarizing them with DAG graphics which illustrated the specific statistics of the GO data.The analysis of the GO data using AmoG01.8 showed a higher ratio of GO enrichment related to organic acid metabolic processes,cellular component organization or biogenesis,other organisms,photosystems,nucleoside-triphosphatase activity and oxidoreductase activity in the M9 strain,while the ratio and absolute number of GO enrichment related to protein locolization is higher in the wild type of Cucumis melo L.cv Hetao.Finally,based on the Log2Foldchange values of the transcriptomes,we screened out 85 genes that showed great differences between the two strains during the fruit ripening process using statistic tools such as R Language.9 of 85 genes were further picked out to provide data and evidence for further studies.