| Leaf angle and leaf width of maize are important factors of plant type.Three 4th generation populations constructed with cross between three different pairs of inbred lines are taken as materials in this research.Leaf angle of the first,second and third leaf above ear are measured,and genetic model analysis is conducted.Ye478 and Qi319 as well as 300 RILs taking them as parents are also used as populations.The angle of the first leaf above and under the ear,and the width of the leaves around the ear are measured.QTL of the leaf width and leaf angle are identified by a genetic linkage map with 152 SSR markers,and SNP genetic map is adopted to identify the QTL results of leaf width.The main research results are as follows:1.Taken Zheng58×Chang7-2,C8605-2×598 and CN165×81162 as three groups of parents to construct three 4trh generation populatiois,single separate generation analysis is adopted to sdtudy the genetic rules of the leaf angle.The angle of three leaves above ear of population from Zheng 58×Chang 7-2 and the third leaf of population from C8605-2×598 are in consistent with model E-6,namely mixed inheritance model of two pairs of codominant major genes plus polygenes with additive-dominant effects;and all the three leaves above ear of parent CN165×81162 and the first leaf and second leaf above ear of parent C8605-2×598 are in consistent with model E-1,namely mixed inheritance model of two pairs of major genes with additive-dominant-epistatic effects plus polygenes with additive-dominant effects.The second leaf above ear from Zheng58 × Chang7-2,the first leaf and the third leaf above ear from C8605-2×598,and all three leaves above ear form CN165×81162 show high heredity of the major genes,with an average rate above 70%,and they are insusceptible to the environment and could be chosen as traits for plant architecture.2.Parent Ye478 and Qi319 as well as RIL populations are planted in two different environments,Shijiazhuang and Xinxiang.And the leaf angle of the first leaf above ear and that of the first leaf under ear are measured.Eight QTL relevant to the leaf angle of the first leaf above ear is gained with SSR genetic map,which distribute on Chromosome 1,2,6 and 10,explaining 5.47%?20.01%of phenotypic variance,and one consensus QTL is detected on Chromosome 1.Five QTL relevant to the leaf angle under ear are gained,which distributed on Chromosome 1,5,6 and 7,explaining 5.59%?9.20%of phenotypic variance,and one consensus QTL exists on Chromosome 6.3.52 QTL relevant to maize leaf width of the first leaf above ear,ear leaf and the first leaf under ear are detected in four environments with genetic map containing 152 SSR markers,in which 17 are related to leaf width of the first leaf above ear,15 related to the ear leaf and 20 to the first leaf under ear.The markers distribute on Chromosome 1,2,4,6,7,and 8,and there are 26 QTLs with phenotypic variance rate over 10%.One QTL locate between Marker bnlg1808 and dupssr9 on Chromosome 7,which detects all the three traits in four environments,explaining 18.36%of phenotypic variance,and the genetic distance of the two markers is 9.33cM.According to Sequence B73,the physical distance between the two markers is 10.1Mb,and that is identical with the previous researches.4.The locationing result of SNP genetic map shows that there is one consensus section on Chromosome 7 simultaneously controlling the leaf width of the first leaf above ear,the ear leaf and the first leaf under ear,and the section is identical with the locationing results of the SSR map,with the physical distance shortening to 1.2Mb.It also shows that there exists one consensus section on Chromosome 1,4 and 6 simultaneously controlling the leaf width of those three ears,whose location is approximate to that of the SSR location. |
PDF Full Text Request