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Studies On New Type Plant Disease Resistance Inducer Of FBT To Soil-Born Disease

Posted on:2018-01-18Degree:MasterType:Thesis
Country:ChinaCandidate:S H ChenFull Text:PDF
GTID:2323330515462261Subject:Pesticides
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Effective prevention and control of diseases is an important part of plant protection research.it is a promising method to induce systemic resistance by inducing factors Under the premise of sustainable development of agriculture.Soil borne disease is always a difficult problem to restrict the agricultural development of our country,for our land borne diseases seriously and no simple effective way to control problems,a new type of resistance in this paper with independent intellectual property rights of the inducer of fluconazole activated ester(2,2,2-three and[1,2,3]two fluoride ethyl benzene thiopHene-7-triazole ester,FBT)as the research object to explore the application of technology of fluorine activated ester induced plant resistance soil borne diseases,the activated ester poison media technique of fluconazole in vitro activity,study the application technology of application of pot culture method,through the activation of fluconazole ester induced changes of POD,PPO,Cucumber after PAL and MDA content on the induced resistance mechanism the study also explored with cucumber as the safety of fluoride was activated ester material,the main results are as followings:In vitro and in vivo activity of methyl ester.5%fluconazole activated ester EC no bactericidal activity in vitro,the active ingredients in 15 mg/L and 25 mg/L concentration,5%fluconazole activated ester EC on the rice seedling blight?cucumber damp-off?corn sheath bligh 3 types of Maize Sheath Blight Disease Control effect were 31.88%?61.91%,56.52%?88.71%and 39.08%?63.220%.The experiments show that the spray of fluconazole activated ester can induce plant disease resistance,and provides an effective way of foliar application methods for disease control.Clear the application of the technology of activated ester.Through pot experiment showed that 5%fluconazole activated ester EC at concentration of 25 mg/L,7 days apart after 3 Application on plant induced resistance,application of fluconazole activated ester induced maize sheath blight.The results showed that the duration of 15 days or more.The mechanism of the resistance of the methyl ester.Cucumber after fluconazole induced by active ester without inoculation and inducing inoculation treatment,inoculation control treatment and water control treatment,different treatment showed that cucumber induced after the inoculation in a certain period of time can make PAL,PPO,POD activity increased,the content of cucumber leaves in MDA was lower than control.Fluconazole activated ester whether inoculation or not,can increase cucumber leaves by PAL,PPO,POD activity,and can reduce the content of MDA,inducing inoculation and inoculation of pathogens can be controlled in a certain period of time when PAL,PPO,POD activity increased more obviously and the MDA content significantly decreased.The results indicated that the activation of methyl ester could improve the activity of plant defense enzymes and decrease the content of MAD.The safety of the ester was determined.Fluconazole dose spray activated ester of 15 effective components of 25 and 50 mg/L and root irrigation treatment on cucumber seedling height,intemode length,fresh weight and dry weight have different degrees of inhibition;in a dose of 15 effective components,25 mg/L spraying or pouring root treatment on the cucumber seedling and stem diameter the root shoot ratio of the role,but the dose 50 mg/L of stem diameter and root shoot ratio showed inhibition.The test showed that fluconazole activated ester has good effect on induced resistance of cucumber Damping off disease,and has a certain effect on the cucumber seedling pHysiological indexes.
Keywords/Search Tags:methyl ester, soil borne disease, induced disease resistance, resistance mechanism, pHysiological index
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