Studying On The Expression Pattern Of Egg-typ Aromatase Gene In Ovary Development Of Miiuy

Cytochrome Aromatase P450 is a key enzyme in the synthesis of estradiol,which catalyzes the conversion of androgen to estrogen,which is lower in male gonads.The use of aromatase inhibitors or methyl testosterone can inhibit the expression of aromatase.In the study of fish found in two different genes encoding p450 arom,respectively,from the gonads and brain.These two aromatase have specific promoter and different tissue distribution,express by different regulatory factors,in embryonic development process has different time and space expression,has a unique function.In the whole aromatase reaction process,cytochrome p450 aromatase plays a leading role.Therefore,it is of great significance to carry out the study of aromatase.Ovarian aromatase in follicular granulosa cells,promote hormone and granule cell receptor binding,stimulate granulocyte growth,and promote granule cells to synthesize aromatase.However,granulosa itself can not synthesize male hormones,but luteinizing hormone and endometrial cells combined to promote the cells will acetic acid and cholesterol into androstenedione,androstenedione by diffusion into the granulosa cells,granules in the cell was aromatic to form a female female hormone is secreted into the follicles and blood,and thus to promote the role of follicular development.In the experimental study of the fish,we found that the aromatase in the March-March fish can detect the expression of aromatase in time and space,in its blood can also be detected estradiol,which shows that fish In the early development of gonadal has been initially with steroid synthesis polymerization capacity.And we found that the activity of aromatase in fish was gradually enhanced with the development of ovaries.It is through the catalytic conversion of male hormones into estrogen,prompting the liver to synthesize egg yolk.In conclusion,we believe that the egg-type aromatase can promote the formation and accumulation of yolk in the process of ovarian development,so that the development of the egg can be carried out normally,which plays an important role in the ovary development of the fish.