The Proteomic Analysis Regarding The Effects Of Grafting On The Yield Of Cassava Storage Roots

Cassava(Manihot esculenta Crantz)was originated in tropical America.It is shrubby perennial and belongs to Euphorbiaceae family and Manihot genus.It was widely cultivated in tropical regions around the world.Cassava was introduced into China in 1820s,now widely distributed in Southern China.Cassava is an important dietary source after rice and maize,it is the staple food for 800 million people in the world.In 2014,China's cassava planting area was 380 thousand hectares,and fresh cassava production were 7.8 million tons with about 20 tons per hectare.There is still much growth spacecompared to the theoretical output of 90 tons per hectare.Therefore,it is necessary to study the factors that affect the yield of cassava storage roots,and to provide theoretical basis for the further improvement of cassava yield.cassava genome was highly heterozygous,and traits of spring were serious,and self incompatibility,cross breeding efficiency is low.It is very difficult to improve the yield of cassava by only using the traditional cross breeding method.Grafting as an agricultural technology plays an important role in improvement of plant breeding.Grafting of cassava not only can keep the fine traits of the stock varieties but also can use the favorable characteristics of the scionsIn this study,Mosaic-leaf Mutation and Cultivar ZM-Seaside were as the materials,through two kinds of cassava grafting each other,and proteomics research,to investigate the effect of grafting on yield and protein of Cassava.Reveal the main reason for this yield differences from the angle of whole protein,find the key proteins affecting cassava yield.To provide basic data for the selection of high-yield cassava varieties.The results are as follows:1.Photosynthetic parameters of leaves were measured using a portable analyzer LI-6400.The results showed leaf Pn in ZM-Seaside was significantly increased more than that of mosaic-leaf mutation,while Cs,Ci and Tr in both genotypes were no significant differences.The result showed that ZM-Seaside photosynthetic efficiency was significantly higher than that of Mosaic-leaf mutation.2.The expression levels of Rubisco,OEC and PRXQ,related with photosynthetic pathway,UGPase,SPS,AGPase,related to starch synthesis,in ZM-Seaside was significantly higher than that of mosaic-leaf mutation.3.ZM-Seaside root yield is significantly higher than that of mosaic-leaf mutation.After grafting,the yield of graft plant using ZM-Seaside as rootstock,mosaic-leaf mutation as scion were significantly decreased,and the yield of graft plant using mosaic-leaf mutation as rootstock,ZM-Seaside as scion were significantly increased.4.There was no significant change in the content of hydrocyanic acid and crude starch in grafting and non-grafting.5.Twenty differential protein spots were detected in the leaves of ZM-Seaside compared with mosaic-leaf mutation,of which 15 were up-regulated,5 was down-regulated.Thirty-nine differentially expressed protein spots were detected in the storage root of ZM-Seaside compared with mosaic-leaf mutation,of which 23 were up-regulated,16 were down-regulated.After grafting,mosaic-leaf mutation was used as control,19 differentially expressed protein spots were detected in the plant using mosaic-leaf mutation as rootstock,and ZM-Seaside as scion,of which 5 were up-regulated,14 was down-regulated.ZM-Seaside was used as control,8 differentially expressed protein spots were detected in the plant using ZM-Seaside as rootstock,and osaic-leaf mutation as scion,of which 1 was up-regulated,and 7 were down-regulated.