The Foundation And Optimization Of Vitro Culture And Rapid Propagation System Of Apple Dwarfing Rootstock

Apple dwarf anvil high-efficient and intensive cultivation mode has become the world's apple industry development direction,short anvil cultivation in China accounts for less than 10%,the lack of dwarf rootstock is one of the causes of this situation,this study apple stock M9,Mac9 and B9,SH6,SH38 and SH40,as the experimental material,studied the tissue culture,rapid propagation,transplanting and transplanting process aimed at improving the apple stock tube seedlings rooting rate and survival rate,improve the apple dwarf rootstock in vitro rapid propagation technology system,for the clone dwarf rootstock breeding to provide technical and theoretical support.The main findings are as follows:The research on the breeding of apple's dwarf root stock,M9,Mac9,B9,SH6,SH40 and SH40 was developed by means of tissue culture.1 The establishment of the implants of the apple's dwarf root stock,M9,Mac9,B9,SH6,SH38 and SH40.Explants disinfection solution using 70% ethanol 30 s + 8 min + 0.1% mercuric chloride sterile water wash 5 times has good disinfection effect.Explant materials the best time for April.In the early generation of the new outings of apple root stock M9,Mac9,B9,SH6,SH38,and SH40;M9,Mac9,B9 is suitable for the first generation medium,which is MS + 6-BA1.0mg/L + IBA0.5 mg/L.SH6,SH38,and SH40 is suitable for the first generation medium,which is MS + 6 – BA0.5 mg/L + IBA0.5 mg/L.2 The expansion of apple's dwarf root stock,M9,Mac9,B9,SH6,SH38,SH40,Propagation mediumand root culture.Apple dwarf rootstock suitable proliferation of M9 medium was MS + 6 – BA0.6 mg/L + IBA 0.5 mg/L,the number of effective new tip M9 6.4 / strains,proliferation of young plants growth in good condition,rooting medium 1/2 MS + M9 suitable IBA1.2 mg/L,rooting rate reached 58.6%;Mac9 suitable proliferation medium was MS + 6 – BA0.8 mg/L + IBA 0.5mg/L,effective new tip number 6.1 / plant,proliferation of young plants growth in good condition,Mac9 suitable rooting medium 1/2 MS + IBA1.3 mg/L+melatonin 0.03mg/L;B9 suitable proliferation medium was MS + 6-BA1.0 mg/L + IBA 0.5 mg/L,the number of effective new tip B9 5.6/strains,proliferation of young plants growth in good condition,B9 suitable rooting medium 1/2 MS + IBA1.0 mg/L+melatonin 0.03mg/L.SH6 suitable proliferation medium was MS + 6 – BA1.0 mg/L + IBA 0.1 mg/L,number 6.8 / plant,and effective new tip young plants growth robust,SH6 appropriate rooting medium 1/2 MS + IBA1.4 mg/L,rooting rate reached 62.7%,respectively.The best breeding medium for the SH38 is MS + 6-BA0.6 mg/L + IBA 0.1 mg/L,and SH38 is suitable for rooting medium 1/2MS +1.4 mg/L.The SH40 suitable proliferative medium is MS + 6-BA0.8 mg/L + IBA 0.1 mg/L,SH40 suitable for rooting medium 1/2MS + IBA1.3mg/L3 Screening of seedling transplant conditions.Apple stock M9,Mac9 and B9SH6,SH38,SH40 and domestication of transplanting conditions as follows: seed MiaoGen more than 3 cm long,number 4 above,seedling height more than 4 cm,at least four leaf seedling;First closed bottle refining blade 6 d,bottle refining plants 3-6 d again,3/5 to 3/5 field soil + vermiculite as matrix,transplanting survival rate were 76.6%,76.6%,77.5%,77.2%,68%,77.2%...