Inhibitory Effects Of Four Plant Polysaccharides On Acute Infection Induced By ALV-J In Chickens

Avian Leukosis（AL）is a combination of avian leukemia virus（Avian Leukosis Virus,ALV）and avian sarcoma virus（Avian Sarcoma Virus,ASV）caused by a variety of avian tumor diseases collectively,in many subgroups,Subgroup J avian leukosis virus（ALV-J）is more pathogenic and transmissive than classical avian leukemia,resulting in more serious economic losses.In recent years,China’s sub-J subgroup of avian leukemia virus（ALV-J）-induced myeloid cell tumors are widely popular at the same time,in some egg flocks and "817" broiler flocks in a certain proportion of the surface Fibrosarcoma,the disease mainly through the innate infection and the level of contact with the spread of infection in the laying hens,broilers and local flocks have occurred in the family,resulting in chicken immunosuppressive,tumor death,secondary infection and other hazards,to the poultry industry Bring huge economic losses.Due to the complexity of the ALV-J gene sequence and its antigenic variability,so far the lack of effective vaccines and drugs can only be controlled by population purification.However,due to poor management of farming,purification is not complete and other reasons,the disease in the poultry area is still widespread,especially in developing countries.Therefore,there is an urgent need to explore new ways to control ALV infection and spread.Taishan pine pollen,Sophora japonica,ginkgo and aloe are gathered a lot of life elements,rich in nutrients and active substances,with antibacterial,anti-virus,anti-oxidation,improve immune function and other functions,and tumor cells and HIV Inhibit the role of human health great benefit.However,it is not clear which of these plant polysaccharides will have the best inhibitory effect on ALV-J-induced tumors,which is important for anti-ALV-J infection and tumor-suppressing drug development.In this study,we collected the freshly collected pine pollen,aloe vera,ginkgo biloba and Sophora japonica with the acute tumorigenic J subgroup avian leukemia virus（ALV-J）as the starting point.Polysaccharide,the effects of four plant polysaccharides on the inhibition of acute tumorigenic ALV-J tumor were studied.The antitumor effect of four kinds of plant polysaccharides was compared and the four kinds of polysaccharides were tested by the related immunological indexes.Effects of plant polysaccharides on the body infected with acute tumorigenic ALV-J chicken.This study was divided into the following two parts:Part One: Effects of four polysaccharides on the growth of ALV-J acute tumorFu-J virus is a kind of ALV-J-related acute tumorigenic virus,which can cause rapid infection of chicken.In order to study the effects of polysaccharide,aloe polysaccharide,ginkgo biloba polysaccharide and Sophora japonica polysaccharide on acute tumorigenic ALV-J In this study,we observed and recorded the clinical symptoms,the time of tumor onset and the natural death period.The results showed that the pine pollen polysaccharide group could significantly inhibit the expression of ALV-J induced by the other groups Tumor growth rate and prolong the natural survival time of chicks.Secondly,in this study,HE staining and immunofluorescence staining were performed on the isolated tumor tissues.The results showed that the lines with tumor tissue and surrounding tissues were clear and the number of tumor cells was low,Tissue virus content and some cells apoptosis.The results showed that the inhibitory effect of pine pollen polysaccharide group on chicken acute tumor was superior to other experimental group.Part Two: Comparison of four kinds of Polysaccharides on immune enhancement of ALV-J acute tumorIn order to investigate the effects of polysaccharides from polysaccharides,aloe polysaccharides,ginkgo biloba polysaccharides and polysaccharides from Sophora japonica polysaccharides on immunization of acute tumor-induced acute tumor-induced acute neoplasms,this study measured the number of CD₄+ and CD₈+ T lymphocytes in the blood,lymphocytes The levels of IFN-γ,IL-2,TNF-α and ALV-J were measured.Through the detection of related immune parameters,we found that the use of polysaccharide after immunization enhancement is better than non-oral polysaccharide PBS control group.At the same time,we also found that the polysaccharide group of polysaccharide was the most significant,the aloe polysaccharide group and the ginkgo biloba polysaccharide group were the second.The total effect of Sophora japonica polysaccharide group was worse than that of the other three groups.The results showed that the polysaccharide group had better immunopotentiation effect on chicks infected with ALV-J acute tumor.The results showed that the polysaccharides of pine pollen could delay the infection time of acute tumorigenic ALV-J chickens and prolong the natural survival time of infected chicks and inhibit the growth of chicks.In addition,the related immunological indexes The results showed that the polysaccharide had good immunopotentiation effect on the infected chicken,and it was better than that of aloe polysaccharide,ginkgo biloba polysaccharide and Sophora japonica polysaccharide.