In Vitro Regeneration Of Diploid Actinidia And Polyploid Induction

In this study,the diploid Actinidia: the hybrid strain HM,SM and Qi Hong were used as materials,firstly,utilizing the shoots of HM,the leaves of QH and the leaves of SM to established rapid propagation system.Then explored the optimum conditions to obtain polyploidy ploid by colchicine induction technology.The main results were as follows:Established the rapid propagation system with the diploid plants HM and obtained the most appropriate medium.The most appropriate medium for regeneration was MS+3.5 mg/L 6-BA+1.0 mg/L NAA+7.5 g agar +30 g sucrose;The optimum medium for bud proliferation and differentiation was MS+3.0 mg/L 6-BA+1.0 mg/L NAA+7.5 g agar +30 g sucrose;and the optimum medium for rooting was 1/2 MS+2.0 mg/L IBA+ 7.5 g agar + 30 g sucrose.Induced the shoots of HM by using colchicine,which concentration was 50 mg/L.Successfully obtained 18 tetraploid HM plantlets and 16 plantlets have obvious phenotypic difference.Established the rapid propagation system with the diploid plants QH and obtained the most appropriate medium.The most appropriate medium for regeneration was MS+3.5 mg/L 6-BA+1.0 mg/L NAA+ 7.5 g agar +30 g sucrose;The bud proliferation coefficient was 11.2,the most appropriate medium for bud proliferation and differentiation was MS+3.0 mg/L mg/L NAA+ 7.5 g agar +30 g sucrose,and the most appropriate medium for rooting was 1/2 MS+2.0 mg/L IBA+7.5 g agar +30 g sucrose.Induced the leaves of QH by using colchicine,which concentration were 100 mg/L,150 mg/L.366 induced plantlets were obtained and 55 plantlets have obvious phenotypic difference.Established the rapid propagation system with the diploid plants QH and obtained the most appropriate medium.The most appropriate regeneration medium was MS+2.5 mg/L 6-BA+1.0 mg/L NAA+7.5 g agar +30 g sucrose;The optimum medium for bud proliferation and differentiation was MS+2.5 mg/L mg/L NAA+7.5 g agar +30 g sucrose;and the optimum rooting medium was 1/2 MS+2.5 mg/L IBA+7.5 g agar+30 g sucrose.Induced the Shoot tips of SM by using colchicine,which concentration were 100 mg/L,150 mg/L.366 induced plantlets were obtained.