Cloning And Expression Of β-1,3-glucanase Gene From Bacillus Atrophaeus SF1

In order to explore the function of its antagonist protein,the β-1,3-glucanase gene was cloned and expressed in this paper.The main contents and results of this paper are as follows:1.Cloning of the P-1,3-glucanase gene:The β-1,3-glucanase gene bgl was cloned from Bacillus atrophaeus SF1,gene length 1086 bp,G+C content 50.4%,encoding 358 amino acids,the theoretical molecular weight of 47 kDa.2.Expression of β-1,3-glucanase gene in Escherichia coli:The clonedβ-1,3-glucanase gene fragment was ligated with the expression vector pGEX-4T-1 to construct the recombinant plasmid pGEX-4T-1-bgl,transformed into host strain BL21(DE3),and successfully obtained engineering strain NDM-1.The relative molecular weight of the fusion protein was about 73 kDa by SDS-PAGE.3.The activity and antibacterial effect of β-1,3-glucanase produced by NDM-1 in engineering strain:When the strain NDM-1 was cultured for 24 h,the antibacterial rate reached the highest value of 40.8%,which was the bacteriostatic rate of strain SF1 56.2%.