| Lily is one of the very important cut flowers in ornamental plants of the world,which is of the highest value in flower market.But the number of the pollen of Lily is huge partly because the flowers are large and open to outside,which makes it easily to be polluted by its pollen,but also It is a very time-consuming effort.Therefore,cultivating male sterile Lily has a larger real economy value and potential breeding value.In this research,the plant materials are Male Sterile Lily Variety 'No Anther White' and Its Recombinant Inbred Lines with Anther.High throughput sequencer and bioinformatics tools were implemented to construct on stamens in the different developmental stages of the flower buds.The data obtained from the sequencing were assembled into transcripts,and the functional annotation analysis of the GO?KEGG?eggNOG?SwissProt Kegg Pathway functional enrichment analysis was performed on the obtained Unigene.The main samples are stamens mixed samples at different developmental stages of lily pollen,the results of this study are as follows.The library of stamens was constructed and sequenced by high throughput sequencing platform.The raw data is first filtered,the resulting high quality sequence is spliced,and then the resulting transcript sequence is compared to the database?NR?.The 206942 sequence contig was obtained and 98551 transcripts were obtained.The total number of sequences was 37699 Unigene and the results were clustered to obtain Unigene,GO,KEGG,eggNOG,SwissProt annotation for the obtained Unigene.Anthers Degraded Male Sterile Lily Variety 'No Anther White' and Its Recombinant Inbred Lines with Anther were divided into two groups according to their genera,medium and small,respectively DGE libraries have been constructed based on different development period of stamens.An overall quality evaluation of gene expression has done according to the samples of RPKM value,the results showed that gene expression patterns of the samples is conformed to mRNA heterogeneity and redundancy.The DGE data showed that all stamens has differences on expression,some raised and others get lowed.Finding the profiles of different periods of expression of different genes and clustering expression pattern,which consist of significant enrichment of gene function analysis and estimating the genetic function,and qRT-PCR which these can provide basis for gene identification.Taking the different organs as explains tissue culture and rapid propagation of hybrids'No Anther White'.The results showed that the regeneration ability of different explants varies significantly with different organ.When 2,4-D was 1.5 mg·L-1 and KT was 0.1 mg·L-1 was the best culture media for scales.When 2,4-D was 2.0 mg·L-1 and KT was 0.5 mg·L-1.When 2,4-D was 3.0 mg·L-1 and KT was 0.5 mg·L-1.MS + 6-BA 3.0 mg L-1 + NAA 0.2 mg·L-1 is the best medium for the proliferation of adventitious buds.Good growing at 1/2MS + NAA 0.1 mg·L-1 + IBA 0.1 mg·L-1,good rooting at 1/2MS + NAA 0.1 mg·L-1 + IBA 0.1 mg·L-1,good root number.It is easy to survive for seedlings living in this medium. |
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