Expression Characteristics And Genetic Transformation Of SlSWEET11b In Tomato

SWEET protein is a novel sugar transporter,which plays an important role in the process of sugar transport.Recent studies have shown that SWEET protein is involved in the process of ion transport,growth and development,and adaptation to the environment in Arabidopsis thaliana and rice sexual aspects play an important role.The preliminary study showed that SlSWEET11b gene was expressed in tomato fruit,and the specific role of this gene in tomato fruit development was not clear.In this study,the characteristics of SISWEET11b gene were identified by bioinformatics and real-time quantitative PCR,and the function of SlSWEET11b gene was analyzed by transient expression and stable genetic transformation by constructing overexpression and silencing vector.The main results as follows:1.The length of the SlSWEET11b gene was 1124bp and encoded by 375 amino acids according to the tomato database.The transmembrane structure analysis showed that the gene had seven transmembrane structures and contained two MtN3 domains.The phylogenetic tree analysis of the Arabidopsis thaliana SWEET family showed that the protein was located in Clade ?.According to the functional analysis of other SWEET proteins in the branch,the protein may be responsible for the transport of sucrose and fructose.2.The results showed that the expression level of SlSWEET11b gene reached the peak at 21 days after tomato,and then decreased at the same time.In the fruit ripening process,the expression of the gene is not the same,which in the green fruit period of the glial placenta,the color of the ventricular wall,red fruit sepals and fruit vascular bundle expression is higher.3.The full length of SlSWEET11b gene was successfully cloned,and the expression vector was constructed by double digestion method.Silent expression vector was constructed by Gateway technique.The expression of SlSWEET11b gene was significantly higher than that of the control group(P<0.05).The expression of SlSWEET11b was significantly higher than that of the control group(P<0.05)and the expression of SlSWEET11b gene was significantly decreased after the constructed silencing vector was injected into tomato fruit.The above results indicate that the constructed overexpression vector and silencing vector can affect the expression of SlSWEET11b gene and can be used for further analysis of tomato genetic transformation.4.Stable genetic transformation system was established by using the model plant Micro-Tom tomato.Seven strains of SlSWEET11b were obtained.Four tomato plants were obtained by PCR and analyzed for further functional analysis.