Expression Of GmPRs(Glycine Max Pathogenesis-related Proteins) In The Interactions Between Soybean And Soybean Cyst Nematode And Research Of Callose Deposition

In order to investigate the expression of pathogenesis-related proteins(GmPRs)in soybean roots after inoculated by Heterodera glycines.Six GmPRs genes expression were examined after inoculated by Heterodera glycines in resistant cultivar Huipizhiheidou and susceptible cultivar Liaodou 15 by using real-time quantitative PCR(RT-PCR)technology,it was clear that GmPR2(?-1,3-glucanase)and GmPR10 were associated with soybean resistance to soybean cyst nematode.Furthermore expression vector was constructed to research the subcellular localization of GmPR2 and GmPR10.Study on the resistant and susceptible varieties and hybrid progeny in callose deposition characteristics and the effect of callose in soybean cyst nematode through the method of callose aniline blue staining.And the expression of five soybean callose synthase genes was determined at the transcription level.The main results are as follows:1.In the resistant varieties,the expression of GmPR2 gene was significantly increased at the initial and developmental stages of nematode infection in the early stages of nematode infection and development.There was no significant difference in the expression of susceptible varieties.The expression of GmPR3 and GmPR10 reached the highest value during the development of nematode,indicating that-these genes might be related to the regulation of soybean cyst nematode resistance.2.The transient expression vector was constructed and the target gene was transiently expressed in tobacco leaf by Agrobacterium tumefaciens transformation method.The target gene was located in tobacco epidermal cells by FITC excitator with positive fluorescence microscope.The results showed that GmPR2 was mainly distributed in the cell membrane and GmPR10 mainly distributed in the cytoplasm and nucleus.It was believed that the expression of pathogenic proteins was related to the regulation of transcription,substance transport and extracellular information transmission.3.The results showed that the nematode resistant varieties to produce a large number of early callose deposition by callose staining,Susceptible varieties callose deposition rarely,the trend of resistant hybrids was the same as that of the former.Callose deposition inhibited the growth of nematode.These results suggest that callose production is not just an early indicator of soybean cyst nematode stress in plants but likely to be part of the defense pathway that leads to the inhibition of root growth.4.In resistant and susceptible cultivars,the expression of five callose synthase gene in soybean by callose deposition(GmCALS)were measured at the transcription level in different time after inoculation point.The results showed that the callose synthase gene was induced to varying degrees in the interaction between soybean and SCN.GmCALS7 and GmCALS 10 genes may not be related to the synthesis of callose.GmCALS5 gene was up-regulated and expressed at 3d to 5d after nematode infection.The expression of GmCALS3 gene was up-regulated on day 4 and day 5 after nematode infection.And the expression of GmCALS3 gene and GmCALS5 gene may be related to the synthesis of callose.