| Acremonium terricola culture(ATC) are obtained in Cordyceps feed additive,through processing Acremonium terricolafrom Cordycepsgunniiby artificial solid and liquid fermentation.Acremonium terricola culture possess a similar functional ingredients to natural Cordycepsgunnii,such as cordycepic acid,cordycepin,cordyceps polysaccharide,and has benefit functions of growth promoting,enhance the ability of antioxidation and immunoregulation for animals.Firstly,based on ruminally cannulated multiparous holstein cows experiment,the study estimate effects of Acremonium terricola culture as feed additive,and then verify the application effect in transition cows production,makes a preliminary exploration on its antioxidant and anti-inflammationmechanism.Experiment one: The purpose was to research the effects of Acremonium Terricola culture(ATC)on ruminal fermentation and verify whether it has adverse effects on dairy cows by blood biochemical indicators,which could provide the data base for the following experiment.Four ruminally cannulated multiparous Holstein cows averaging 600 kg of body weight were used in a 4×4 Latin square design.Cows were fed close-up,in the morning and evening 6:00 accurate feeding diet,free drinking water throughout the day.Trials were basal diet group and basal diet+10,20 and 30 g ACT(dry matter basis),each lasting 15 days,sampling period 3 days,a total of 4.Supplementation of ATC could improve concentration of NH3-N,TVFA,acetate and propionate(P<0.01)and regulate relative population size of ruminal microorganism.Supplementation of ATC could improve the concentration of blood glucose and globulin(P<0.01),and reduce the concentration of beta hydroxybutyric acid,non-esterified fatty acid and alanine aminotransferase(P<0.05).Experiment two: The aim of this study was to investigate the effects of ATC on antioxidant capacity,negative energy balance status and production performance of transition dairy cows.We provided a theoretical basis and data support for the future application of ATC in ruminant production.Twenty multiparous Holstein cows were distinguished based on previous lactation mature equivalent milk yield into 2 treatment groups to start the study 14 ± 3 d before the expected calving date and continue through 21 d postpartum.The only different factor in this experiment was the ATC(control: Basal diet;treatment: 30 g ATC of dry matter basis cow-1 day-1 + Basal diet).They were fed a TMR ad libitum once daily.On the day after calving,cows were switched to the appropriate postpartum treatment TMR,which was also fed once daily ad libitum,and the cows had free access to water.The cows supplemented with the ATC produced more milk and had a lower somatic cell score in their milk than the non-supplemented cows(P<0.01).The cows supplemented with ATC also had higher concentrations of superoxide dismutase and glutathione peroxidase and a higher total antioxidant capacity of plasma than the control cows in the perinatal period(P<0.01).In addition,the cows that consumed the ATC had higher blood glucose(P<0.05)and lower ?-hydroxybutyrate and nonesterified fatty acid concentrations(P<0.01)in the plasma;furthermore,the concentration of cholesterol was lower(P<0.05).In conclusion,ATC could improve rumen fermentation,furthermore,improve antioxidant function,production performance and negative energy balance status of perinatal cows.ATC was a kind of high quality feed additives can be widely used in the pasture. |
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