Gene Mapping Of A Narrow Leaf Mutant?dnal1?in Rice

Rice is one of the major food sources of the people in the world,As the world's population continues to increase and the area of cultivated land is decreasing year by year,new challenges are raised for the increase in production.Breeders have been plant breeding as a key research object,especially the leaf morphology research.Up to now,many studies have focused on leaf traits,but narrow leaf traits are also a kind of leaf morphology,and there is also a great impact on yield.For example,narrow leaves make plant populations more reasonable light Its own photosynthesis,and ultimately to improve the biological yield.We obtained a stable and narrow leaf mutant by the radiation restorer line R225.The hybrids of indica rice were used to construct the hybrid combinations,and the F1 hybrids were planted.The F2 phenotype was harvested,and the F2 phenotype was harvested.Plant population.1.Phenotypic Characterization of MutantsThe mutant and wild type were identified at the same time,The mutant plants showed dwarfing characteristics,and the leaves were narrowed obviously.The tiller was more than the wild type,and the leaf length did not change obviously.And wild type phenotypic data analysis found the following results.The mutant height was 77.78 cm,which was 28% shorter than that of the wild type.The mutant flag leaf width was only 0.48 cm,which was 31% of the wild type.The tiller number of the mutant was 21.7,while the number of tillers in the wild type parent was 15.2,the seed setting rate of the mutant was significantly decreased,only62%,the grain length was 95% of the wild type,and the grain width was not significantly different.2.Genetic analysisThe mutant and indica rice cultivars were identified as normal leaves,indicating that the mutant trait was controlled by recessive genes.The F2 population was obtained from the self,and the leaves were broad and narrow.(?2= 0.0105 <?2 0.05,1= 3.84).The results showed that the ratio of normal and mutant strains was 3: 1(?2 =0.0105 <?2 0.05,1 = 3.84).3.Mut Map sequencing preliminary positioningThe initial locus was a Mut Map sequencing scheme,The leaves of the F2 population were divided into two groups,and two DNA-pools were mixed respectively.At the same time,the DNA was extracted from the two parents,and then the two DNAs The results of two DNA-pool sequencing were anchored to 12 chromosomes on the chromosomes,and the SNP-index was screened on the chromosomes,and the SNP-index was screened.The results were as follows: And finally the target gene is located on chromosome 7.4.Screening of candidate genesIn this study,the gene was located on chromosome 7 by improved Mut Map technique.And the gene was located at 4513167-5417953 using the Idel marker developed by sequencing.Two methods were used to screen out the candidate gene.