The Study Of Xanthomonas Campestris Pv.Campestris Effector XC3176 And XC1210 To Regulate Plant Innate Immunity

Plants is activated the PAMP-triggered immune(PTI)response when recognized the pathogen-associated molecular patterns(PAMPs)of phytopathogen.Many phytopathogen can directly transport the effectors into the plant cells through the type? secretion system(T3SS)to suppress the PTI and cause the effector-triggered susceptibility(ETS).At present,the molecular mechanism of many effectors is still unclear.Hence,study their interactions between effectors and plant will help us know more about the mechanism of plant resistance and bacterial virulence.Xanthomonas campestris pv.campestris(Xcc)is one of the most important plant pathogens which can world widely infect cruciferous plants,bringing huge economic losses.Seventeen type ? effector proteins have been identified in Xcc 8004 strain.However,the molecular mechanism of these effectors remain unknown.In this study,we focus on the regulation of plant innate immunity of two virulence-related type ?effect proteins XC3176 and XC1210.Firstly,the subcellular localization of XC3176 and XC1210 were studied by the PEG/Ca mediated Arabidopsis protoplast transient expression system.The results show that XC3176 and XC1210 was located in the cytoplasm and the cell membrane of host cells,respectively.Secondly,the virulence assay shows that the virulence of the deletion mutants of XC3176 and XC1210 significantly reduced in Arabidopsis compared to the wild-type Xcc 8004.For further study the interaction betweenXC3176,XC1210 and the host plant,XC3176 and XC1210 transgenic plants were constructed respectively.It was found thatXC3176 and XC1210 can stable express in Arabidopsis transgenic plants of XC3176 and XC1210 respectively.The callose deposition and the expression of the PTI early defense-related genes,At1g51890,At2g17740,AtFRK1 and At5g57220 were inhibited in both transgenic plants of XC3176 and XC1210.The virulence assay show that whatever Xcc 8004,Pseudomonas syringe pv.tomato DC3000 and AhrcV can infect XC3176 and XC1210 transgenic plants easier than pBI121 transgenic plants.