Screening And Identification Of Antagonistic Bacteria Against Pathogen Of Ginger Blast

The research object of this subject - ginger has a lot of efficacy,not only can be used as medicine, but also has the role of sweating, warm stomach, lungs cough,detoxification, etc.. But with the increase of China’s demand for exports of ginger,ginger in the process of cultivation, easy to infection with Ralstonia solanacearum led ginger blast disease,a serious impact on China’s Ginger quality,caused a lot of ginger total crop failure. Ginger blast is mainly caused by Pseudomonas solanacearum,currently in ginger blast control mainly by physical, chemical and biological control,biological control of the most commonly used method is mainly through effective screening of antagonistic strains, beneficial colonization in ginger rhizome department which has a restrictive effect on ginger blast. But at present, there is no good method for biological control in production. In order to find an effective prevention and control method, this experiment was carried out to screen and identify the resistance to Ralstonia solanacearum. To obtain good control of ginger blast with the expansion,the author on the basis of previous research experience, the isolation and screening of antagonistic rhizosphere ginger blast Ralstonia solanacearum growth promoting bacteria from the rhizosphere soil of Anhui province ginger planting area of diseased plant rhizosphere and healthy ginger, based on the preliminary study on the antagonistic properties, combined with morphology, culture characteristics, species specific sequence analysis, physiological and biochemical characteristics and 16SrRNA identification, classification and identification of antagonistic strains screened, and biological control of ginger blast for functional strains effectively, lay a theoretical foundation for ginger blast control, the main research is as follows:(1)by dilution plate method in solution, Gause’s synthetic agar medium and phosphate-solubilizing bacteria medium and nitrogen-fixing bacteria medium of three kinds of selective medium isolation and screening of antagonistic bacteria,culminating in the cultivation of nitrogen fixing antibacterial screening two strains of antagonistic groups, respectively N3 and N5. Then the two strains of antagonistic bacteria growth and nitrogen fixation competition test to measure their antagonistic ability, showed that strain N3 had better antagonistic effect of ginger wilt, antagonistic ability is two, can effectively inhibit the bacterial wilt of ginger blast pathogen growth;N5 strains of ginger blast bacterial wilt antagonistic effect and antagonistic ability for a compared with N3, antagonistic effect of weak ginger blast.(2)the screening of the N3 and N5 strains were identified by morphological and physiological and biochemical, the test results show that the production of indole acetic acid, indole acetic acid producing strain N3 was higher than that of general strain N5, antagonistic to ginger can promote the growth of plants; soluble phosphorus, total phosphorus solubilizing strain N3 activity is relatively higher than that of N5 strain the formation of compounds, fusion of phosphorus in soil in ginger in ion antagonism can be better combined, not easy to drain, prevention of soil diseases; siderophore producing strains, N3 overall siderophore production capacity is slightly higher than that of N5 strain, can be a good combination of iron pigment supply of low molecular weight substances of microbial cells, effectively promote plant absorption microorganism in soil.(3)the N3 and N5 strains were identified by SDS-K, and the total DNA was extracted and detected by electrophoresis, The bands of 1500bp were obtained by amplification of bacterial gene sequences, and by sequencing N3 and N5 strains were Arthrobacter and Burkholderia..