The Study On The Why Transfomating Arabidopsis And Brassica Napus L. And The Tolerant Responses To Drought And Salt Stress

Drought and salt stress are two major factors that affect plant growth and development,which seriously restrict the development of agriculture.At present,due to the continuous development of genetically modified technology,the use of transgenic technology to develop new varieties of salt and drought-resistant crops has become the focus of national research.Brassica napus L.is an important economic crop in China,and it is also affected by drought and salt stress.Therefore,it is also one of the hot spots to improve B.napus L.resistance to salt and drought resistance by using transgenic technology.WHY is a non-specific binding site present in DR1372 protein from Deinococcus Radiodurans(DR),and a large number of studies have shown that the Why is a domain gene related to water stress,and it is inferred that the transition of the Why into plants may improve the resistance to water stress and reduce the drought and salt damages to plants.In this study,GV3103-Why and EHA105-Why plant expression vectors were constructed by genetic engineering and we got the transgenic Brassica napus L and Arabidopsis thaliana.Under salt stress and drought conditions,Under salt stress and drought conditions,the physiological and biochemical indexes of A.thaliana were determined.Research on why gene for the ability of plant response to drought and salt stress provide s theoretical basis for our plant breeding work.The results showed that the relative germination rate and relative root length of transgenic A.thaliana were significantly higher than that of wild type Arabidopsis under moderate salt stress and moderate drought stress.The relative glycoside content and the relativeconductivity of leaves of transgenic Arabidopsis were significantly lower than wild type,while the content of proline,soluble sugar,soluble protein,chlorophyll and water content were significantly higher than that of wild type.Most important was that antioxidant enzyme activities of transgenic A.thaliana were higher than wild type.The results indicated that transgenic Arabidopsis had stronger salt tolerance and drought tolerance than wild type.From above,the results showed that the Why gene was directly or indirectly involved in the mechanism of drought resistance and improved salt tolerance and obviously in Arabidopsis.