Proteome Research Of Cotton Seeding In Responses To Potassium Deficiency Stress

Potassium is one of the main inorganic nutrition elements in plants.It plays an important role in regulating cell membrane potential,controlling cell osmotic potential,promoting plant photosynthesis and participating in protein synthesis.Cotton is an important economic crop in our country.Potassium deficiency can lead to premature senescence,further seriously affects the yield and quality of cotton.At present,little is known about molecular mechanism of cotton in adaptation to potassium deficiency and the proteins acting in tolerance to potassium starvation,although considerable progress has been achieved in physiological mechanisms for cotton in response to potassium deficiency.In this experiment,the material is upland cotton zhong S9612.We studied the proteomic changes of cotton seedling in response to potassium limitation using the two-dimensional electrophoresis technology.According to the cotyledon and true leaves brown spots phenotypes after potassium deficiency treatments for 12,16,and 20 d respectively,we determined the sampling times.After extracting the total proteins in leaves and roots,respectively,we separated these proteins by the two-dimensional electrophoresis technology,and obtained the difference expression protein pots between potassium deficiency treatments and the control using correlation analysis software.The above results showed that 14,2,36 proteins were up-regulated,and 8,14,21 proteins were down-regulated in cotton true leaves for 12,16,and 20 potassium deficiency treatments respectively.In cotton roots for 12,16,and 20 potassium starvation treatments,7,63,57 proteins were up-regulated and 33,5,0 proteins were down-regulated,respectively.We successfully identified 101 differential expression proteins according to their mass spectrum information in cotton leave and roots.Through the UniProt database,we obtained the function annotation of these differentially expressed proteins,and classified these proteins due to their functions.The classification results showed that the differentially expressed proteins can be divided into 10 kinds according to their biological functions.These include metabolism,protein synthesis,oxidation,stress response,photosynthesis,signal transduction,acid-base balance,cell differentiation,transcription pathways and regulation of biological clock.We examined the levels of gene expression of some differentially expressed protein related genes,and found that the expression of only individual genes increased,and that of most genes was not affected by potassium deficiency treatments at the three different time points.Together,these data will lay a foundation for us to further investigate the mechanisms for cotton responding to potassium deficiency.