| Hyriopsis cumingii is native only to China.Meanwhile,it is the most important pearl mussel in China.In this study,microsatellite markers were developed from transcriptome data of H.cumingii,and multiple PCR sets were established.The studies on stock genetic differentiation,growth performance and inner shell color inheritance were carried out using the molecular marker developed.The primary results were as follows:1.Development of EST-SSR marker and establishment of multiplex PCR for H.cumingiiA total of 110 pairs of microsatellite marker were selected from Transcriptome data of H.cumingii,and then we obtained 38 polymorphic EST-SSR markers.The number of alleles(Na)ranged from 3 to 23 with an average of 8.47.The observed heterozygosity(Ho)ranged from 0.313 to 1.000 with an average of 0.731 and the expected heterozygosity(He)from 0.360 to 0.910 with an average of 0.711.Nine EST-SSR markers were found to be moderately polymorphic(0.25 < PIC < 0.5)and the remaining ones were highly polymorphic(PIC > 0.5).None of the 38 loci showed a significant deviation from Hardy-Weinberg equilibrium(P < 0.05).Three multiplex PCR panels including 12 polymorphic EST-SSRs were optimized by Multiplex Manager version 1.0.2.Comparison of growth and genetic performance of different inner shell color groups of H.cumingiiThe cultured stock of H.Cumingii reproduced,and in their offspring there are various colors appeared in inner shell,such as purple and white and yellow.At the age of 3 months and 13 months,the growth traits and shell color were measured,respectively.Our results revealed that,at the age of 3 months,there are no difference inshell length,shell width and body mass among three kinds of shell color groups showed no growth advantage.However,at age of 13 months,the shell length,shell width and body mass of the purple and white stocks were faster than that of the yellow stock,.But there was no significant difference in growth performance between the white and purple stocks.Meanwhile,the new established three groups of multiplex PCR panels were used to conduct the genetic analysis of three kinds of inner shell color groups.Our results demonstrated that the observed heterozygosity(Ho)of yellow stock was larger than that of the white and purple stocks.The expected heterozygosity(He)and polymorphism information content(PIC)of the purple stock were the highest in the three stocks.Moreover the genetic diversity of the purple stocks is the highest and that of the white stock was the lowest.We used MEGA.4 software to construct phylogenetic tree.We found that the genetic distance between the white and yellow stock was the smallest,and that between the purple and yellow stock was the largest.In the phylogenetic diagram,the white groups and yellow groups clustered together first and then clustered with the purple groups.3.Analysis on the genetic law of inner shell color of H.cumingiiThe white and purple inner shell color individuals were selected from two color strains of H.cumingii as experimental parent samples for 2 x 2 diallel cross experiment.Our results showed that the inner shell color of H.cumingii was inherited stably.The purple inner shell color trait was dominant over the white color,and the two kinds of color traits were not maternal inheritance.It was forecasted that the white and purple color traits of the inner shell of H.cumingii was a quantitative trait controlled by one major gene,and the expression of the shell color was affected by the environment. |
PDF Full Text Request