Effects Of The Oviposition Of Saperda Populnea On The Expression In Auxinrelated Genes Of Populus Cv Xiaohei

Saperda populnea L.is a herbivorous borer,which has a greater harm to the poplar clumps,and often grows larvae in the trunk after ovulation.In recent years,studies on herbaceous plants have shown that plants are affectde by the insect pests,the formation of gall is reated to auxin.Three types of transmembrane proteins are known to control the transmembrane movement of auxins: PINs,ABCBs and AUX / LAXs.The three family genes are most expressed in the developing xylem,but there is no report that they regulated by the genes involved in the formation of gall yet.In this study,the changes of the morphological and hormonal contents of the damaged parts of Populus ussuriensis were studied by fluorescence quantitative PCR and in situ RT-PCR analysis.The expression of auxin transport protein was detected by fluorescence quantitative PCR and in situ RT-PCR In order to reveal the effect of the hormones on the gall in the oviposition of Populus simonii.At the same time,the method of mechanical injury was used to analyze the mode of transport of hormones during the formation of the gall in the cows.The relationship between the formation of plant itself and the formation of gills and the growth hormone.The main results are as follows: 1.The morphological organization of the injury site of the Saperda populnea L.spwning in the Populus×xiaohei T.S.Hwang et LiangAfter the persecution of Saperda populnea L.spawning in the Populus×xiaohei T.S.Hwang et Liang,department of the phloem damage with the extension of time,and the color will gradually deepen from dark brown to brown,to dark brown,to black,and then the callus will gradually form.Slices observed that phloem cells were squeezed together,the cell morphology became long,the cell contents contracted,the color intensified,the cells lost their activity,and finally the trees were self-repairing,growing near the edge of the victim Callus.2.Effects of Saperda populnea L.spawning on the content of hormones in Populus×xiaohei T.S.Hwang et LiangThe content of hormones in the bark of the damaged parts of the beetles and the damaged buds of damaged shoots were measured.The contents of IAA and GA3 in the bark and bud were the highest,which was consistent with the growth of plant stem and bud.The auxin in the shoots was basically increased,while the auxin in the bark was the first drop and then rise.The content of hormones in the bark was higher than that in the terminal buds,indicating that the repair of the damaged parts and the formation of the galls were related to the auxin.3.The expression of auxin transport protein genes after the Saperda populnea L.spawning in the Populus×xiaohei T.S.Hwang et Liang.Twelve PIN family genes,eight ABCB family genes and eight AUX / LAX family genes were screened by polyacrylamide electrophoresis.Fluorescence quantitative PCR showed that the eggs could decrease the expression of auxin-related genes in the barkof the Populus×xiaohei T.S.Hwang et Liang within 24 hours and then part of the genes will gradual recovery.The results of in situ RT-PCR and morphological observation showed that the recovery of partial gene expression was related to the formation of new callus.4.A Preliminary Study on the Transport Mode of Hormones after Damage to Populus×xiaohei T.S.Hwang et LiangThe callus of 2-year-old Populus×xiaohei T.S.Hwang et Liang was observed.After 5 days,the new callus was observed in the control group and at the site of injury,and 30μM NAP and 30μM TIBA were added to the ring.There was a small amount of callus at the ring cutting,and no obvious callus was formed at the lesion.The damage repair of the black poplar requires the polar transport of auxin.