Genetic Analysis And Molecular Marker For Architecture Traits In Melon(Cucumis Melo L.)

Melon?Cucumis melo L.2n=2x=24?,which is belongs to the Cucumis of Cucurbitaceae family,is an annual trailing herb that widely cultivated around the world and has a high diversity.In recent years,cultivation area of melon,especially the area of protected cultivation is constantlly growing.Reasonable architecture is an important factor for production of protected cultivation.So reasonable plant architect-ture breeding for protected cultivation of melon become more and more important.In this study,the compact-type variety B103 and the loose-type B297 were used as parent lines and the F₁ and F₂ populations were then constructed.The mixed major gene plus polygene inheritance mod-el was used to investigate the inheritance of internode length and petiole length and leaf area and leaf stalk-stem angle.While using SSR molecular mark and bulked segregant analysis combined to mark the F₂ population.Looking for molecular markers linked to melon architecture traits which using molecular marker linkage analysis.Main results are as follows:The results showed that only the leaf stalk-stem angle had the same genetic model in two years.Its inheritance was controlled by a mixed genetic model of two major genes with additive-dominant-epistatic effects plus polygene with additive-dominant effects?the E-1 model?.The other there traits?internode length and petiole length and leaf area?were not in the same genetic model in two years.The inheritance of internode length was controlled by a mixed genetic model of two major genes with additive-dominant effects plus polygene with additive-dominant effects?the E-2 model?in the autumn of 2015 and was controlled by a mixed genetic model of additive-dominant polygene with additive-dominant effects?the E-6 model?in the spring of 2016.The inheritance of petiole length was controlled by a mixed genetic model of two major genes with equal additive effects plus polygene with additive-dominant effects?the E-4 model?in 2015 and was controlled by additive-dominant-epistatic polygene mode?the C-0 model?in 2016.The inheritance of leaf area was controlled by additive-dominant-epistatic polygene model?the C-0 model?in 2015 and was controlled by a mixed genetic model of two major genes with equal additive effects plus polygene with additive-dominant effects?the E-4 model?in the 2016.We can learnt that the four traits major controlled by genetic but also influenced by environ-mental factors.Among the 326 SSR primers tested,98 were polymorphic between B103 and B297.The poly-merphism rate was 30.06%.The BSA method was used to establish eight gene pools according to the field survey result of F₂ generation populations.Then 98 SSR primers tested in the 8 mixed pools and amplificated in 134 F₂ populations.Finally,we got four SSR markers associated with internode length,two of them were linked,the distance between markers was 17.5cM.Two SSR markers were related to petiole length,the distance between markers were 35.6cM.There were two SSR markers related to leaf area but did not linked.Five SSR markers were related to leaf stalk-stem angle,the distance between markers were 35.6cM.