Functional Analysis Of OsLPR1 On Rice Growth And Phosphate Utilization

Phosphorus is one of key macro nutrients repuired for plant growth and metabolism.Due to Pi(PO43-,HPO42-,H2PO4-)is easy to be fixed,and its movtion ability in the soil is bad,which makes the content of soluble phosphorus in saturated soil solution is very low(less than 10 uM),far from meeting the need of plant growth.So whether plant can use a small amount of soluble phosphorus efficiency in soil has a crucial effect on plant growth.In order to adapt to low phosphorus in the soil conditions,the plants in the long-term evolution process,many adaptation mechanisms in physiological and biochemical have been evolved,including changing the struction of root system,secrete organic acids by roots,and combine mycorrhizal fungi form mycorrhizal symbionts,induced expression of phosphate transporters,and a series of molecular regulate mechanism of response phosphorus signal.Previous studies has clearly reported that LPR1/2(LOW PHOSPHATE ROOT1/2)is a ferroxidase localized in the cell walls and endoplasmic reticulum in Arabidopsis,involved in the the primary root development and mechanism of the sensing the in the environment under the lack of phosphorus condition.As endogen model plant,Rice has many different from dicotyledonous model plant Arabidopsis in root development and the response mechanism of phosphorus.Through the multiple genome database retrieval,five LPR genes have been identified in rice,according to their sequence with the AtLPR1 consistency from high to low,named OsLPR1-OsLPR5 in turn.We have an in-depth study on the LPR1,the main results were as follows:1.The gene sequence accession number of OsLPRl is Os01g0126100,full-length gene sequences have 2413 bp,including 3 exons and 2 introns,and the length of its transcription mRNA sequence is 1749 bp,encode 582 amino acids.The consistency of amino acid sequences of OsLPR1 with AtLPR1/AtLPR2 were 57%/55.3%,respectively.2.Through the quantitative RT-PCR analysis showed that OsLPRl gene is mainly expressed in rice root and shoot base,it also has a small amount of expression in leaf;in addition,the expression of OsLPR1 was found to be significantly induced when lack of K、Fe,but decreased when lack of N>P.3.OsLPR1 was mainly expressed in ovary,embryo,root,collar and node through the OsLPR1pro::GUS transgenetic plant.4.Through the subcellular localization analysis,OsLPR1 is localized in endoplasmic reticulum and nuclear.5.OsLPR1 affect the seed germination and plant growth.When knock down OSLPR1,seed germinate slowly in earlier stage;when OsLPR1 was overexpressed,seed germinate slowly,and the plant height,root length and biomass decreased significantly.6.OsLPRl involved in Pi homeostasis and distribution of rice.Pi concentrations in the shoot of OsLPR1-Ox plants were significant increased,It may suggest OsLPRl affect the transport and redistribution of Pi in plant cell.7.Through the study of the relationship between OsLPR1 gene and nutrition,it provides a preliminary basis for the research and development of new fertilizer.