Determination Of Production Performance And Analysis Of Single Nucleotide Polymorphisms Of TYR?DCT Gene In Black Mutant Raccoon Dog

Raccoon dog is a precious fur-bearing animal and coat color is an important economic trait determing quality and value due to use of its fur as economic animal fur.In recent years,the black mutant raccoon dog(black raccoon dog)has been found in the breed of raccoon dogs,as a new mutation,because of its pure,bright color coat,it has great value in scientific research and utilization.As a new mutation,its basic research is still blank,so figuring out the biological characteristics of black raccoon,and investigation of mechanisms about hair pigmentation will provide important theoretical and practical significance in the process of cultivating new varieties of coat color for raccoon dog.The growth performance,growth performance,fur performance and blood physiological and biochemical indexes of black raccoon dog were determined.Selected TYR,DCT genes which Potentially affected the black raccoon dog coat colors,reveals the cause of the black mutations at the molecular level.The main results were as follows:1.The growth performance,growth performance,fur performance and blood physiological and biochemical indexes of black raccoon dog were determined,while the normal raccoon dog was for comparison.The results showed that there was no significant difference in the litter size and survival rate between black raccoon dog and normal raccoon dog in the same feeding and management conditions(P>0.05).In the growth performance,the body weights of 45-210 days were no significant difference(P>0.05).In the fur performance,the length of needle and villi of black raccoon was significantly higher than that of normal raccoon dog(P<0.05).The blood physiological index and biochemical index were measured,and the data were compared.In Physiological indexes,PLT in the male was significant higher than that in female for the black raccoon(P < 0.05);W-SCR in raccoon dogNyctereutes ussurienusis was significant higher than that in black male raccoon dog(P < 0.05).Other results WBC,RBC,HGB,HCT,MCV,MCH,MCHC,W-SCC,RDW-SD,RDW-CV,PDW,MPV,P-LCR,were not significant different between breeds and gender.In Biochemical indexes,the differences of TG related to gender,especially,TG in raccoon dog Nyctereutes ussurienusis was significant between male and female(P < 0.05).Alb,TP,CHO,there were not differences between the two kinds of raccoon dog(P > 0.05).ALT,AST,Glu,Urea,TP,D-HDL-C,LDH,CHO,ALP were no significant difference in interspecific and gender.2.The TYR,DCT gene complete coding sequences of black raccoon dog and wusuli raccoon dog were got by PCR,cloning sequencing technologies,of The two genes were identified and bioinformatics analysed through comparative genomics.According to the results,TYR gene coding sequence is 1593 bp long,encoding 530 amino acids.DCT gene coding sequence is 1650 bp long,complete encoding 549 amino acids.3.By sequencing analysis results,detecting the SNPs in TYR,DCT gene encoding area,analysis the correlation of the genotypes of gene mutations and raccoon coats phenotypic.Sequencing and comparison results show that the raccoon TYR gene in a mutation loci c.1497 c > G,only exist in the black raccoon dog and the mutations for CG genotypes,wusuli raccoon dog for CC genotype,the mutation were associated with black raccoon dog coat formation;DCT gene coding region in black raccoon dog and wusuli raccoon dog have no mutation between the two kinds of colors.