Application On Rapid Diagnosis Of Bovine Mastitis And Exploration On Diagnostic Indicators Of Sub-clinical Bovine Mastitis

Bovine mastitis has plagued the development of dairy farming,is one of the most important diseases of dairy industry.There are many factors that lead to inflammation of dairy cow breast,but most of them are caused by infection of pathogenic microorganism.In the identification of pathogenic microorganisms,isolation and identification are the earliest and most authoritative methods.Somatic cell count(SCC)is an indicator of health status in dairy cow breast.In recent years,a variety of new pathogen identification technologies have been applied to the identification of pathogenic bacteria of bovine mastitis,at the same time,the new diagnostic index of mastitis was explored.In this study,we investigated the prevalence of mastitis in dairy cows in Henan Province,and identified the pathogen by using the methods of separation and identification as well as the microfluidic chip,then milk acute phase protein,rennin,milk composition of milk with mastitis were analyzed,and blood routine analysis was performed on diseased dairy cow for exploring the effect of the prevalence of mastitis in dairy cows and the mastitis on milk acute phase protein,rennin,milk composition and blood routine,thus providing references for new indicator indexes.In this process,we tried to develop the application software in order to improve the efficiency of diagnosis and provide better services for the ranch.The main contents of this study are as follows:First research: The prevalence of mastitis in dairy cows in Henan province was investigated.Based on the DHI report,CMT and De Lava portable cell analyzer were used for collecting 185 mastitis samples from 27 ranches in the province,pathogen was identified by the methods of separation and identification as well as the microfluidic chip.Results showed: the highest detection rate of pathogens were Streptococcus uberis(40.20%),Corynebacterium bovis(26.47%),Streptococcus agalactiae(11.76%),Escherichia coli(6.21%),Mycoplasma(6.21%),etc,among them,S.uberis,C.bovis and E.coli are environmental pathogenic microorganisms;S.agalactiae and Mycoplasma are infectious pathogens.Results suggested: recession of dairy industry led to the elimination of weak and sick dairy cows,which brought the improvement of the mass production performance and diseases resistance,infectious pathogens have been controlled,but still need to be vigilant.The higher detection rate of the environmental pathogenic microorganisms are needed to improve by the further feeding management.Second research: in order to explore the new diagnostic indicators of sub-clinical bovine mastitis,the effect of sub-clinical bovine mastitis to APP,enzyme,milk composition and blood routine are explored,and the correlation analysis with SCC is performed.According to the ranch DHI report,the ranch with higher monthly average of SCC was selected,and CMT was referred,De Lava portable cell analyzer was used for screening 20 dairy cows whose SCC<50×104/m L,28 dairy cows whose SCC>50×104/m L,milk and blood of them were taken for analyzing.The research indicated: The contents of HP,SAA,AGP and CRP between two groups were significantly positively correlated with SCC(P<0.01),and the correlation coefficients(R)were: HP(r=0.806),SAA(r=0.662),AGP(r=0.604),CRP(r=0.717),respectively.The activities of LDH,MPO,NAG and CAT between two groups were significantly positively correlated with SCC(P<0.01),and the correlation coefficients(R)were: LDH(r=0.729),MPO(r=0.433),NAG(r=0.932),CAT(r=0.430),respectively.There was significant difference in the lactose content before and after the occurrence of mastitis(P<0.01),which was significantly positively correlated with SCC(r=0.765,P<0.01).The mean corpuscular hemoglobin concentration(MCHC)in blood was positively correlated with SCC(r=0.413,P <0.01).Results suggested: The contents of HP,SAA,AGP and CRP in milk may be used as the diagnostic indexes of mastitis in dairy cows;the activities of LDH,MPO,NAG and CAT in milk may be used as the diagnostic indexes of mastitis in dairy cows;however,the relativities of LDH and NAG were the highest,the specific diagnostic indicatrix still needs to be further explored.There are many factors affecting milk composition,it varies a lot between domestic and foreign reports,in this study,lactose can be used as a reference for the diagnosis of mastitis,but further research is still needed.There are few studies on the changes of blood routine before and after mastitis in dairy cows.Whether MCHC can be used as indicator of bovine mastitis remains to be explored.Third research: Research and development of information diagnosis platform of dairy cows.In the process of investigating the prevalence of mastitis in dairy cows in Henan Province,a set of “bovine mastitis test report generation system” was developed in order to improve the efficiency of mastitis detection.Research content: “bovine mastitis test report generation system” can be based on the detection results of the input automatically points out the corresponding prevention and treatment advice,and generate the detection report."Dairy cow expert system" bases on the actual disease condition of dairy cows in the production and combined with relevant literature information,thus forming the management system for dairy cow disease diagnosis,which is used for diagnosing the dairy cow disease according to the input information of users.The above research aims to achieve the diagnosis of common diseases of dairy cows to facilitate the timely and rapid diagnosis for the technology staff in the ranch,which not only helps the dairy farmers,but also promotes the healthy development of dairy industry.