Subgroup J Avian Leukosis Virus NX0101 Strain Gp85 Gene Mutation Analysis In Different Organs In The Same Chicken

Avian leukosis is one of the main diseases of causing poultry tumor,whose pathogen is avian leukosis virus(ALV)of a retrovirus genus.According to the viral envelope glycoprotein antigenic structure,host range and mutual interference between different strains cultured in cells,ALV can be divided into 10 subgroups A to J,and subgroups A,B,C,D,E and J exist in chickens.Subgroups A,B and J are the main exogenous ALV that cause chicken tumors,but it is rare to hear tumors caused by subgroups C,D.Subgroup E is endogenous leukosis virus,which has low or no pathogenicity for chickens.In the last century before the 80's,avain leukosis virus subgroups A,B are the two viruses that caused chicken lymphoid leukemia and various tumors,but in the middle of the 80's,international large breeder companies have basically eliminated the infection of ALV-A and ALV-B.In recent decades,China has never carried out any nationwide purification measures against ALV,exogenous ALV infection most likely exist in chicken flocks in China,especially the local strains.In recent years,chicken/leukemia cases have encountered with many times,chicken leukosis has been one of the important diseases that harm the poultry industy in our country.Zhu Mei-zhen.ect isolated a strain of ALV-A from Shandong local strains,Zhao Dong-min.ect isolated a subgroup B avian leukosis virus from chickens of Chinese native breed luhua.It shows that chicken in our country have presence of A,B subgroup avian leukosis virus infection.To research the gp85 gene mutation of subgroup J avian leukosis virus NX0101 strain in different organs,different tumor tissue of broiler.Select a broiler breeder of diffuse tumors especially in liver,kidney,lung.DF1 were inoculated in leaching liquid of the left kidney large tumor,liver,right kidney,lung,IFA testing,DNA were extracted from different organs(left kidney large tumor,liver,right kidney,lung),PCR,pick 10 positive clones from each organ,then sequenced,have gp85 homology comparison with the original virus NX0101.Inoculation strain NX0101,left kidney large tumor,liver,right kidney,lung virus gp85 gene average homology were 99.75%,95.6%,94.9%,95.7%,94.4%compare with the original virus NX0101.inoculation strain NX0101,left kidney large tumor own,liver,right kidney,lung themselves 10 clones homology were 99.5%,99.3%,98.7%,99.2%,98.9%.Left kidney large tumor clones homologous were 96.1%,98.1%,96%compare to liver,right kidney,pulmonary clones,liver clones in the homology of 96.3%and 96.5%compare to right kidney and pulmonary clones,the homology was 95.8%between renal and pulmonary clones.Inoculation strain NX0101 10 clones have 8 stable mutations compared to original strain.The NS/S values in the entire gp85 and 110?120aa,141?151aa and 189?194aa three hypervariable region were below 2.5.But clones in different organs have 31 stable mutations,and most stable mutation sites were in three hypervariable region,in the entire gp85 the NS/S values of clones in different organs were between 2.83 and 7.29,in three hypervariable region the NS/S values were as high as 10 and even more than 10.NX0101 gp85 gene have lhigh variability when copy in the various organs in the same body,but in different organs its variation degree is different,even in the same organs,dfferent clones gp85 homology also has the certain differences.In different organs gp85 gene has larger difference than in the same organ.Avian leukosis virus infection diversity showed not only in the groups,while in individual infection among different organs,there also had obvious diversity.