Analysis On Gene Expression Profile Of Chrysoperla Sinica(Neuroptera:Chrysopidae) Diapause Under Photoperiodic Control And The Effect Of Diapause On Offspring Development

Chrysoperla sinica?Tjeder?is an important natural enemy.It is of great value to study the diapause status as overwintering stage.To clarity the molecular mechanism of Chrysoperla sinica diapause and the effect of diapause to Chrysoperla sinica growth and offspring population,and transcriptome sequencing data of Chrysoperla sinica adults under long and short photoperiods and the effect of different diapause experience on Chrysoperla sinica adults growth and offsprings populations were analysed preliminarily,in order to lay the foundations for artificial fecundation and application of Chrysoperla sinica.1 About 150 million cleanreads were gotten after transcriptome sequencing through 3 d old C.Sinica adults under long and short photoperiods.Sequence denovo assembly of all clean reads of transcriptome sequencing data obtained 89512 Unigenes and annotated 27914 genes.Lots of differently expressed genes?493?were indentified under long and short photoperiods,in which 109 genes expression under short photoperiod were up-regulated and 384 genes expression were down-regulation.The differently expressed genes were mainly related to DNA synthesis,metabolism of carbohydrate and lipid,and synthesis of vitellogenin.There were significant differences among the expression of G14351,G29643,G36603 and G1164in different diapause periods?short photoperiod treated 3 d,10 d,20 d,30 d and 40 d?,except G9837.2 Different diapause induction periods have effect on the post-diapause development indexes of Chrysoperla sinica?Tjeder?adults.With the short photoperiod treated time extending,the total pre-oviposition period was longer,the pre-oviposition,oviposition period and fecundity were increased at first and then decreased.The pre-oviposition period of adults under 20 d short photoperiod was the longest.The oviposition period of adults under 10 d short photoperiod was the longest.The fecundity of 10 d short photoperiod treated adults was the highest,and the fecundity of 5 d,10 d,20 d short photoperiod treated adults were higher than control group.Different diapause induction period had no effect on Chrysoperla sinica?Tjeder?adults.The diapause intensity of short photoperiod treated Chrysoperla sinica?Tjeder?adults was the highest which was treated 20 d under short photoperiod at 25?,a little time diapause induction??20 d?can improve the fecundity of Chrysoperla sinica?Tjeder?.3 To investigate the effect of maternal diapause experience on the development of Chrysoperla sinica?Tjeder?offspring,the biological characteristics of C.sinica offspring under long and short photoperiods were analyzed by two-sexual life table.Maternal diapause experience had no significant effects on developmental duration and population parameters of C.Sinica offspring under long photoperiod.Diapause experience had no significant effects on developmental duration of C.Sinica offspring under short photoperiod.The pre-oviposition and oviposition period of 50 d short photoperiod treated offspring were significantly longer than the other treatments,and the fecundity was significantly higher.The intrinsic rate of increase?r?,finite rate of increase???,net reproductive rate?R₀?of 50 d short photoperiod treated offspring were significantly higher than the other treatments,but the mean generation time?T?was significantly lower.The results show:different short photoperiod treated time had little effect on the development of C.Sinica offspring under long photoperiod.The longer maternal diapause experience had reduction effect on the diapause induction of C.Sinica offspring,and improve the fecundity off offspring..