Association Analysis And Gene Prediction Of Different Mineral Nutrients Content In Wheat Grain

Mineral elements which are essential for the growth and development of wheat are indispensable nutrient substance for human beings.The wheat mutants of mineral nutrients are an important carrier for studying the genetic control mechanism of grain nutrient content.In this study,17 related traits of wheat on mineral nutrients were investigated using Yannong15 and 102 mutant lines obtained by EMS mutagenesis of Yannong15 by the combination of field cultivation and indoor analysis,which could be detected some associated sites and unigene by the SNP molecular marker genetic map and unigene map to provide stable related markers and candidate genes for wheat breeding in future.The main results were as follows:1 Compared with yannong 15,there were significant variations in the eight mineral nutrient content of wheat mutants with the average variation range from 12.06%to 36.73%,in which the largest and smallest variation were GZnC and GMgC.Compared with yannong 15,The mutants with the range from 0 to 8.82%decreased significantly,and the range from 2.94to 40.20%increased.2 24 typical wheat mineral nutrient mutants were selected in the experiment,in which,M88 is high P mutant;M105,M76,M65 are high Ca mutants;M31,M36 are high Mg mutants;M45 is high Fe mutant;M14,M37,M110,M49,M52,M60,M32,M66 and M29,M75 are high Mn and low Mn mutants,respectively;M93,M7,M68,M100,M97 and M111are high Cu and low Cu mutants,respectively;M74 is high Zn mutant.3 According to the variation of wheat mutants,both of the environment and genotype had an influence on mineral nutrients,of which the impact range was 7.75%to 68.45%and31.54%to 92.25%,which indicates that the variation is mainly caused by genotypic difference.4 For association study of the mineral nutrients using 39481 markers,we detected 10005markers significantly associated with 8 elements at the level of P<0.001,and these were located on the 21 chromosomes.Single marker loci phenotypic variation ranged from 10.34%to 85.70%.5 2387 markers were selected as stable loci at same element in four environments which were expressed in GKC and GMnA with the highest correlation of 2.74E-06 and the contribution rate of 61.05%.Among the significant loci,19 loci which were significantly associated with 9 traits were clustered with the range of P value from 4.74E-07 to 9.96E-04and R~2 from 10.62%to 57.49%.These loci are mainly associated with GPA,GKA,GMgA,GCuA,GMnA,GKC,GPC,GMnC,indicating that multiple traits are controlled by the same gene and can explain the genetic association between quantitative traits.6 Taking the SNP of 19 cluster sites as the focus,a total of 15 candidate genes were predicted based on the location of unigene,the genes with 60%were located on the 1AL,2AL and 3DL chromosomes,which may be involved in the genetic control process of multiple traits and deserve further study.