Characterization And Regulatory Functions Of SOCS1 And SOCS3 Gene In Miiuycroaker

Vertebrates rely on the immune system to eliminate infectious pathogens,when they are suffering the invasion of microbes and pathogens,and the immune system consists of innate and acquired immunity.The innate immune system,as the most universal form of host defense,is the first line of defense against invading pathogens.The suppressor of cytokine signalling(SOCS)proteins were first described as inhibitors of cytokine signaling and had a vital role in the immune systems.Since the first member of SOCS family was discovered,8 SOCS family members were found in mammals,including CISH,SOCS1-7 of SOCS family.However,there were several fish-unique members have been identified in fish,including SOCS1 b,SOCS3b,SOCS5 b,SOCS8 and SOCS9.SOCS1 and SOCS3 have been widely studied in mammals,but the studies of SOCS1 and SOCS3 were still poor.In this study,we identified the SOCS1 a,SOCS1b,SOCS3 a and SOCS3 b genes of miiuy croaker.Then,the characterization,expression pattern analysis and functions of these 4 genes were studied.(1)The full-length of mmSOCS1 a was 3111 bp,including 2 exons and 1 introns.Compared with the SOCS1 a of other species,SOCS1 a has a highly conserved central SH2 domain and a relatively conservative C-terminal SOCS box domain.Gene synteny analysis showed that mmSOCS1 a has a highly conserved synteny with other fish.The expression pattern analysis showed that the expression of mmSOCS1 a was the highest in the liver and spleen,and in the infection experiment,the SOCS1 a was highly expressed in the liver,kidney and macrophage.Double luciferase reporter assays results showed that mmSOCS1 a could inhibit poly(I:C)-,IFN?-and IFN?-induced ISRE activation.(2)The mmSOCS1 b was 3780 bp in length and included 3 exons and 2 introns.Compared with the SOCS1 b of other species,SOCS1 b only has a highly conserved central SH2 domain.Gene synteny analysis showed that mmSOCS1 b has a highly conserved synteny with other fish.The expression pattern analysis showed that the expression level of mm SOCS1 b in the spleen and intestine was the highest.In the infection experiment,SOCS1 b was highly expressed in the spleen and kidney.Double luciferase reporter assays results showed that mmSOCS1 b could inhibit the activition of ISRE induced by IFN? and IFN?.(3)The full-length of mmSOCS3 a was 2576 bp,including 2 exons and 1 introns.By comparing with the SOCS3 a other species,SOCS3 a has a highly conserved central SH2 domain,a relatively conservative C terminal SOCS box domain and a variable N end region.Gene synteny analysis showed that mmSOCS3 a has a highly conserved synteny with other fish.The expression pattern analysis showed that the expression of mmSOCS3 a was the highest in the liver and skin,and in the infection experiment,the SOCS3 a was highly expressed in the liver,kidney and spleen.Double luciferase reporter assays results showed that mmSOCS3 a could inhibit the activition of ISRE induced by IFN?.(4)The mmSOCS3 b was 2686 bp in length and included 3 exons and 2 introns.By comparing with the SOCS3 b of other species,SOCS3 b has a highly conserved central SH2 domain,a relatively conservative C terminal SOCS box domain and a variable N end region.Gene synteny analysis showed that mmSOCS3 b has a highly conserved synteny with other fish.The expression pattern analysis showed that the expression of SOCS3 b was the highest in the liver and kidney,and in the infection experiment,the SOCS3 b was highly expressed in the spleen,kidney and spleen.Double luciferase reporter assays results showed that mmSOCS3 b could inhibit IFN?-induced ISRE activation.