Mapping QTL For Salt Tolerance And Differential Expression Proteins Of Drought Resistance In Maize Seedlings

According as the decreasing of cultivated land area and the increase of population in recent years,the grain will become a problem for humanity,crop production has become the important way to solve the problem of food.In nowadays,it is not so easily by increasing the per mu yield of crop.The development and utilization of land resources is one of important way to improve food crops.Undeveloped land generally has high salt content,or get the disadvantage of water sufficient irrigation.Through the use of saline-alkali land and the droughts land and continued to expand the corn cultivation area is to increase the output of a good solution,corn is sensitive to salt stress and drought stress,especially in seedling stage,cultivate salt-tolerant and drought-tolerant maize varieties can improve the utilization of land and improve maize yield.So,corn breeding about resistance to salt and drought tolerant has a important significance to improve the utilization rate of salt and drought.The main research results were as follows:(1)The 198 F2 population of 8723xP138 and 174 SSR markers were used in construction of genetic linkage map covering 10 chromosomes of maize.The total length of the map was 2764.3 cM,with average genetic distance of 15.88 cM.Through the analysis of phenotype data,we have four traits QTL mapping,Nine QTLs related to salt tolerance traits were detected in the corn seedling.Tree loci related to the relative electrical conductivity,which located in 6 and 9 chromosomes.One was associated with plant height,located on chromosome 2.Three related to the number of root,which located in chromosome 2 and 4.(2)PEG simulated drought stress experiment in seed germination and seedling germination to maize inbred lines Chang7-2,Langhuang and TS141.To verify the conclusions that Chang 7-2 and Lang has higher drought Verify that Chang 7-2 and Langhuang have high drought tolerance,and chang 7-2 is the best,TS141 have a poor ability to drought.(3)Proteomic analysis revealed that 47 proteins were increased and 46 proteinswere decreased in Chang7-2,27 proteins were increased and 22 proteins were decreased in TS141 in response to drought stress.Analysis of differential protein functions reveals that its main function is in binding and catalytic activity,mainly involved in metabolic processes and cellular processes.Phenylpropan-oid biosynthesis,Glycolysis/Gluconeogenesi and Ribosome has a direct or indirect influence on drought resistance of maize.