Based On Transcriptome Sequencing Preliminary Study On Mechanism Of Resistance To Wheat Stem Rust

Stem rust of wheat is caused by wheat stem rust fungus?Puccicinia graminis Pers.f.sp.tritici?,which can be spread in long distance by upper airflow across nationwide or even intercontinental agricultural production regions.The disease has a long history,the literature record can be traced back as far as to the ancient times,people worship"rust god"pray for safety of wheat harvest;Its prevention and control measures have a long history,which can be traced back to the time of the ancient Greek city-states.People completely eradicated barberry shrubs and other shrubs around the wheat fields to prevent the outbreak of stem rust.interestingly.Berberis was later confirmed to stem rust and wheat stripe rust was important to turn the main host,to stem rust finish sexual generation,provide important living environment,and the stem rust sexual reproduction heritage mutation sites,so as to generate new virulent race groups of.Therefore,LC is very suitable for studying the histological and molecular mechanism of wheat stalk rust infection.Wheat straw rusts infected wheat 3 days to form,complete the infection stage;Infection of wheat in 6 days to complete the production of spore,stem rust disease stage began.Therefore,we selected 34MKGQM bacterial strain of small wheat stalk with the highest virulence frequency in China to be inoculated with LC,and the transcriptome sequencing study was carried out for 3 days and 6 days after the inoculation of LC,and the results were as follows:1.The transcriptome sequencing revealed 114429 significant difference genes,of which the GO function analysis,3 days after LC inoculation,24 functional genes were activated.After six days of LC,26 functional genes were activated,both of which were detected by the GO functional analysis:the functional genes including plant hormone signaling,transport function,cell composition and biosynthesis.2.Metabolic pathway analysis:30 metabolic pathways were activated in 3 days after LC inoculation,and 35 metabolic pathways were activated after 6 days.These two groups include hormone metabolism pathway,nitrogen metabolism pathway.Among them,there are 5hormonessignificantlyup-regulatedinthehormonesignalingpathway(log₂^FC>log₂^1.5,log₂^FC<log₂^0.5,p<0.05),ET,IAA,ABA,GA₃,and BR respectively.3.qRT-PCR was used to verify that the sequencing trend of 5 hormone samples and transcriptome was up-regulated.Exogenous spraying inoculation test with fungi on five kinds of hormones,according to the results of ET,IAA,ABA,and distilled water group,positive control group infect"4",GA₃,BR and negative control SA group infect"3",ET,IAA and ABA in regulating stem rust resistance of wheat,GA₃,BR can enhance wheat stem rust resistance.4.Phylogenetic tree showed that wheat AMT protein was homologous with rice AMT protein,tomato AMT protein and arabidopsis thaliana.Among them,the wheat AMT protein was the most homologous with the rice AMT protein,suggesting that the wheat AMT gene could also play a role in regulating the transportation of NH₄⁺capacity in wheat.qRT-PCR test showed TaAMT1 in wheat root,stem,leaf and flower tissue.TaAMT1;1a,TaAMT1;1b,TaAMT1;3a expression is the most abundant,while other TaAMT gene expression level is very low.Minnie 2761 and LC inoculation detection,found from 0h to 48h in LC TaAMT1;1a,TaAMT1;1b,TaAMT1;3a significantly increased,Minnie 2761 TaAMT1;1a,TaAMT1;1b,TaAMT1;3a are no obvious changing.The nitrogen and nitrogen free tests showed that the rust fungus of wheat stalk was significantly reduced from"4"to"3"in the absence of nitrogen.These studies indicated that the expression of TaAMT1 maybe induce the infection of wheat stem rust and negatively regulate the resistance of wheat to stem rust.