| BackgoundHydrogen sulfide is found to be the third gaseous signal molecule in the body found in themammals, following carbon monoxide and nitric oxide, which involved in a variety of physiological and pathological activities and plays an important roles. The expression of hydrogen sulfide can be detected in many mammalian tissues and organs. Many recent studies have shown that hydrogen sulfide is involved a variety of obstructive disease, and there is also experimental verification that the hydrogen sulfide has a protective effect on renal fibrosis. However, the exact role of hydrogen sulfide in the kidney fibrosis is still not clear.Chronic kidney disease is a widespread disease that features gradually decreased glomerular filtration rate, which would eventually lead to renal failure. Renal interstitial fibrosis is the major reason for chronic kidney disease, because of the accumulation of extracellular matrix and renal interstitial myofibroblast proliferation and other reasons. Unilateral ureteral obstruction(UUO) is a typical model of tubular interstitial fibrosis, this model represents the most representative features of obstructive nephropathy, including cell infiltration, tubular cell proliferation and apoptosis, and epithelial-mesenchymal transition(EMT), a fibrotic accumulation of extracellular matrix(ECM) deposition, and renal tubular atrophy. And this disease model has been used in many studies to evaluate the mechanisms involved in these processes. Because UUO model can cause complete obstruction of the kidney ureter, but completely blocked kidney ureter is rarely found in the human body, so this is a disadvantage of Unilateral ureteral obstruction.Autophagy is a catabolic mechanism that involves breakdown of cellular components and recycling of cellular components proteins and organelles within the cytoplasm are allowed to wrap themselves into the vesicles, and vesicles then fused with lysosomes, and targeted cytoplasmic contents are degraded.Studies have shown that autophagy plays an important role in renal fibrosis. Objective:To investigate the protective effects of exogenous hydrogen sulfide(H2S) on unilateral ureteral obstruction(UUO) induced renal injury and explore the possible potential mechanisms involved in this animal model. Methods:Eight weeks old male C57BL/6 mice were randomly divided into three groups, the mice from H2 S group(n=5) were injected one time with Na HS(50umol/day) intraperitoneally(i.p.) for7 days, while the mice from control group were administrated(i.p.) with the same amount of saline(n=5). The tubulointerstitial injury, interstitial fibrosis, and the LC-3and beclin-1 in the kidney were assessed. Density of Cystatin C in the serum was also measured. Results:1. RNA expression of hydrogen sulfide producing enzymes reduced after Unilateral ureteral obstruction.2. Collagen fibers in UUO treated mouse kidney aggregate more than the hydrogen sulfide-treated group.3. Compared with the UUO mice, mice treated with hydrogen sulfide in the experimental group of mice kidney, and the expression of fibronectin is less.4. Compared with the sham-operated mice, mice treated with hydrogen sulfide express more LC-II protein than experimental group; and LC3-II expression in the experimental group compared with mice hydrogen sulfide LC3-II expression of mice to be more. Conclusion:Interstitial fibrosis and renal injury significantly increased after unilateral ureteral obstruction. NaHS significantly ameliorate kidney injury. In addition, LC-3 and beclin-1 in kidney were significantly higher in UUO kidneys compared with that in sham group. The results imply that NaHS significantly ameliorate kidney fibrosis by increase the level of autophagy. H2 S could significantly ameliorate kidney fibrosis by increase the level of autophagy. |
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