Effects Of Pulsed Electric-Magnetic Field On Intracellular Calcium Ions And Cell Membrane Potential In Human Ovarian Cancer SKOV3 Cells

Objective:To explore the effect of pulsed electric-magnetic field (8HZ, 200mt) on the ovarian cancer cell line SKOV3.The effects of PEMF(pulsed electric-magnetic field) on intracellular calcium ions and cell membrane potential were studied.Methods:The cells were divided into three groups:control group, PEMF group, PEMF+verapamil group.The cells in control group did not receive any treatment,cells in the other two groups were exposed to PEMF 1h,50umol/L verapamil was given to PEMF+verapamil group. Calcium ions was labeled by fluorescence probe Fluo-3/AM and membrane potential was labeled by fluorescence probe DiBAC4(3), then laser confocal microscope was utilized to detect the changes of mean fluorescence intensity; Annexin V/PI combined flow cytometry was used to assay apoptosis.Results:Fluo-3/AM fluorescence intensity of the cells increased after exposed to PEMF and the changes were statistically significant compared with the control group (p< 0.05). The fluorescence intensity decreased significantly after the treatment of verapamil when compared to that in PEMF group. DiBAC4(3) Fluorescence intensity of the cells increased after treatment with PEMF (p<0.05), indicating the depolarization of cell membrane; verapamil exerted no remarkable effect on the depolarization of cell membrane (p>0.05). The apotosis rates between PEMF group and control group were not significantly different (p>0.05).Conclusion:PEMF cause cell membrane potential to depolarize, and then voltage-dependent calcium channels are opened, finally increased intracellular calcium level. PEMF did not induce apoptosis under our experiment conditions.