Research On The Anti-breast Cancer Mechanism Of Chickpea Synthetic Peptide CPe-Ⅲ-S

Human health has been threatened by cancer for a long time. Chemotherapy is one of the main methods applied in malignant tumor treatment. Although the effect of killing cancer cells is significant, it has bad influence on the immune function. In recent years, the research for effective active ingredients from natural products has become the focus of cancer treatment. With the development of information science and molecular biology, inhibition mechanism of the tumor by natural products has caused the attention of many scholars at home and abroad.The study used chickpea synthetic peptide CPe-Ⅲ-S as experiment material, through chemical simulation system, tested the total reducing power, OH, DPPH and ABTS radical removal ability. CPe-Ⅲ-S showed strong free radical scavenging ability and ferric reducing antioxidant power with a significant dose-response relationship. The MTT test and p53 protein Elisa experiment showed that CPe-Ⅲ-S had the ability of inhibiting tumor cell proliferation in vitro and promoted the expression content of p53 protein.Modern nutrition research found that protein was digested by enzyme in human digestive tract, and then mainly absorbed in the form of oligo-peptide. Firstly, the study simulated the human gut system in vitro digestion and detected the metabolism. The experiment got three main digestive metabolites: His-Phe-, Ser-His-, Ala-Asn-Ala-Gln-. Secondly, after the in vivo metabolism experiment was adopted to explore the metabolites of the digestive tract. Due to the complexity of environment in the body, there determinates 16 kinds of digestive products, one of the biggest response value is Ser-His-.Molecular docking and molecular dynamics simulation for scientific research provide an important reference to guide experiments and make some theoretical hypothesis, so as to promote the development of theory and experiment. This study analyses the conformation through docking with mutual p53 protein and normal p53 protein, explore the mechanism of inhibiting tumor cell proliferation by CPe-Ⅲ-S and digestive fragments. The results indicate that CPe-Ⅲ-S and their digestive fragments may inhibit cell cancer expression through the combination with mutant p53 protein or may increase the stability of p53 protein by combining with normal p53 protein.