| The roots of Rosa davurica is the root of dahurian rose fruit, which is a rosaceous plant, also known as wild rose root. It is growed in the Northeast(Greater Khingan,Lesser Khingan. etal), North China(Shanxi, Inner Mongolia.etal)and other places.the dahurian rose fruit is very rich in nutrition with a sour and sweet taste and a broad range of use, however, the study on the various chemical components and pharmacologic action of the roots of Rosa davurica is scarce, And the polyhydroxy groups in tannin allow it to well clear the free radical, thus, this paper has studied the content of tannin in the roots of Rosa davurica and antioxidant activity in vitro and vivo, which not only provided a scientific basis for the development and exploitation of resource of the roots of Rosa davurica, but also was very significant to the further production of natural antioxidant.In this paper, the tannin in the roots of Rosa davurica has been extracted, the casein method and HPLC method have been used to measure the content of tannin. The measured result of casein method:among the acetone extract, the overall content of tannin was 62.32%, among the 20% methanol elution parts, the overall content of tannin was 4.74%, among the 40% methanol elution parts, the overall content of tannin was 59.25%. among the 60% methanol elution parts, the overall content of tannin was 24.14%. among the 100% methanol elution parts, the overall content of tannin was 6.43%. the measured result of HPLC method: among the acetone extracts, the content of gallate accounted for 56.37%, among the 20% methanol elution parts, the content of gallate accounted for 3.95%. among the 40% methanol elution parts, the content of gallate accounted for 50.43%. among the 60% methanol elution parts, the content of gallate accounted for 20.68%. among the 100% methanol elution parts, the content of gallate accounted for 4.67%. The results determine the 40% methanol elution parts tannin content was the highest quality, and was used in research of antioxidant activity experiment.In vitro experiments. Vc was used as control group, to explore the effects of tannins in the roots of Rosa davurica in the scavenging action on hydroxyl radical, superoxide anion free radical and DPPH free radical and its inhibiting effect on nitrosation. It was shown by the result of scavenging action on hydroxyl radical that, both of the tannin of the roots of Rosa davurica and Vc can effectively scavenge the hydroxyl radical, within the active mass concentration range of 0.1-0.9mg/mL, the scavenging ability was enhanced as the mass concentration increased, the scavenging ability of Vc had been slightly greater than that of tannin of the roots of Rosa davurica. It was shown by the result of scavenging action on superoxide anion free radical that, within the active mass concentration range of 0.1-0.9mg/mL, the scavenging ability of both the tannin of the roots of Rosa davurica and Vc was enhanced as the mass concentration increased, the scavenging ability of Vc had always been slightly greater than that of tannin of the roots of Rosa davurica.In the experiment of scavenging DPPH free radicals, within the active mass concentration range of 0.1-0.9μg/mL, the scavenging ability of both the tannin of the roots of Rosa davurica and Vc was enhanced as the mass concentration increased, the scavenging ability of tannin of the roots of Rosa davurica was greater than that of Vc, in the experiment of inhibiting effect on nitrosation, the sampling volume was within the range of 1-10ml, as the dosage increased, the blocking ability and scavenging ability of both tannin of the roots of Rosa davurica and Vc had been enhanced with the tannin of the roots of Rosa davurica preceding the Vc. When the dosage of extracts reached to 10ml, the blocking rate peaked at 77.95%, the scavenging rate peaked at 82.08%.The in vivo experiment has investigated the antioxidation effect of tannin of the roots of Rosa davurica on the D-galactose aging mice. The male mice had been selected and successively injected with D-galactose for 4 weeks except for the mice in blank group, which was injected with equivalent amount of normal saline instead, thus, a subacutely aging model of mice has been built, after which, the Vc was taken as a positive control group, the mice in the groups with a high, moderate and low dose of extracting solution received an intragastric administration with a dose respectively as 300mg/(kg-d),200mg/(kg-d) and 100mg/(kg-d), the D-galactose group and blank group received an intragastric administration with equivalent dose of normal saline.In 4 weeks after the experiment, the blood was drawn from the eyeball of mice to measure the content of superoxide dismutase(SOD) and malonaldehyde(MDA) in the serum. It was shown by the result that, the content of SOD in the serum of mice in D-galactose group clearly went down, while the content of MDA clearly went up, the difference between the D-galactose group and blank group was extremely statistically significant (P<0.01); the activity of SOD in serum went up and the content of MDA went down upon the intragastric administration of extracting solution of tannin of the roots of Rosa davurica, compared with the control group of model, the difference in activity of SOD and content of MDA of the moderate dose group were both presenting a extremely statistical significance(P<0.01); the activity of SOD of low dose group extremely significantly went up(P<0.01), content of MDA significantly went down(P< 0.05); the activity of SOD of high dose group significantly went up(P<0.05), the difference in MDA content was not statistically significant; as for the Vc control group, the activity of SOD in serum was enhanced, the content of MDA went down, the difference between the D- galactose model control group and it was extremely statistically significant (P<0.01); the antioxidant ability of Vc group was the strongest, the moderate dose group among the solution of tannin of the roots of Rosa davurica had the best antioxidant effect on the aging mice.It can be seen from the above experiments that, the tannin of the roots of Rosa davurica had a certain antioxidant ability regardless of in vitro or in vivo, which is very significant to seeking a natural non-toxic antioxidant. |
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