Study On Screening Of Pharmacological Activities Of Total Alkaloids Of Selaginella Uncinata(Desv.) Spring

Objective:To study the anti-inflammatory,hypoglycemic,hepatoprotective and anti-tumor effects of the total alkaloids of Selaginella uncinata?Desv.?Spring?TAS?;and then to investigate its anti-tumor mechanism.Methods:?1?Xylene-induced auricle tumefaction and impaired abdominal capillary permeability in mice were used to evaluate the anti-inflammatory effect of TAS.?2?Hyperglycemic mice respectively induced by streptozotocin,adrenaline and glucose were used to evaluate the hypoglycemic effect of TAS.?3?Acute liver injury mice induced by CCl4 were used to evaluate the hepatoprotection effect of TAS.?4?S180,hella and 7404 tumor cells were cultivated in vitro,and then MTT was used to evaluate the anti-rumor effect of TAS.?5?Tumor-bearing mice?S180?were used to evaluate the anti-tumor effect in vivo of TAS.?6?The concentrations of NF-?B and COX-2 in the tumor tissue fluid in mice were determined to investigate the antitumor mechanism of TAS.Results:?1?Either the degree of auricle tumefaction or the impaired abdominal capillary permeability of the high-and medium-dose groups(300,150mg·kg^-1)were statistically less than the control group?P<0.01 or P<0.05?,which indicates that TAS can inhibit the auricle inflammation induced by xylene and abdomina inflammation induced by glacial acetic acid.The anti-inflammatory effect is correlated to the dosage of TAS,showing positive dose-dependent response.?2?Either the ALT level or the AST level were statistically less than the acute liver injury group?P<0.01 or P<0.05?;on the contrary,the TP level and the ALB level were statistically higher,which indicates that TAS can protect the liver acutely injured by CCl4.?3?All the high-and medium-dose groups(1000,500mg·kg^-1·d-1)showed a certain hypoglycemic effect.But the dosage is so high that TAS could not be thought the hypoglycemic component in Selaginella uncinata?Desv.?Spring.?4?TAS showed tumor-inhibition effects not only on the tumor cells of S180,hella and 7404 in vitro,but also on the solid tumors in vivo.The concentrations of NF-?B and COX-2 in TAS groups were statistically higher than the non-drug group,which indicated that TAS can inhibit the expression of NF-?B and COX-2,so then curb or suppress tumor growth.In other words,NF-?B and COX-2 are probably the target point of TAS.Conclusion:?1?TAS is the first times to be extracted from Selaginella uncinata?Desv.?Spring,and to be studied on pharmacological activities.?2?TAS shows remarkable anti-inflammatory,hypoglycemic,hepatoprotective and anti-tumor effects;on contrary,it ambiguously shows hypoglycemic effect.?3?It is presumed that the tumor-inhibition mechanism of TAS is to inhibit the expression of NF-?B and COX-2,so then to inhibit the grow of the blood vessel around the tumor,at last to curb or suppress tumor growth.