| Background Spinal Cord Ischemia-Reperfusion Injury(SCII)is a common multiple factors involved in Spinal Cord tissue caused by the destruction of the progressive,self-destructive cascade amplifying appear even irreversible Spinal Cord neurons late-onset the phenomenon of death.In traumatic surgery,vascular surgery,spinal surgery in areas such as direct injury or surgical complications,SCII is the important cause of secondary spinal cord injury and paraplegia.Always experiments show that fat BML-111 oxygen element agonists(5(S),6(R),7-trihydroxyheptanoic acid methyl ester,C8H16O5)have clear nerve protective effect on cerebral ischemic injuries,to belong to the spinal cord tissue,whether it also play a role of protection,and whether this effect is associated with inhibition of nerve cell apoptosis,has not yet reported.To make a clear definition of the pharmacological effects of BML-111 and to provide experimental basis for clinical treatment.Objective Through the establishment of the rat spinal cord ischemia-reperfusion model,to further explore the impact of lipoxin agonist BML-111 on inflammatory factor and apoptosis in rat spinal cord ischemia-reperfusion injury.Methods Taking healthy adult male SD rats were randomly divided into sham group,ischemia-reperfusion group and BML-111 group.After the two groups of rats by clamping the abdominal aorta method to establish rat model of spinal cord ischemia-reperfusion injury.Ischemia-reperfusion group and the BML-111 rats were administered 30 min after modeling intravenous injection of 0.1 mL of saline and 1 mg/kgBML-111.Results1.Spinal cord function after ischemia-reperfusion rats modeling BBB scores were significantly lower than the sham group(P<0.05);compared with the ischemia-reperfusion group,BML-111 group rats modeling BBB score increased(P<0.05),indicating that BML-111 spinal cord injury repair function acts.2.Spinal cord tissue morphology sham group structure clear spinal cord of rats,neurons large and polygonal nuclei clear center,abundant cytoplasm;ischemia-reperfusion spinal cord of rats apparent infiltration of inflammatory cells,neurons swell nucleus condensation,Nissl indifferent,vacuolar changes in cytoplasm,blood vessels and tissue substance scattered hemorrhage,compared with the ischemia-reperfusion group,the rat spinal cord BML-111 group,pathological changes significantly reduced.3.Spinal cord tissue apoptosis in sham-operated rats rare apoptotic cells;spinal cord ischemia-reperfusion group seen a large number of apoptotic cells in rats;the number of rat spinal cord tissue BML-111 group than in ischemia-reperfusion apoptosis small group(P<0.05).4.Tumor necrosis factor-a and interleukin-1?expression in ischemia-reperfusion group and the BML-111 rats spinal cord tumor necrosis factor a and interleukin-1(3 were significantly increased than sham group(P<0.05),and the BML-111 rats spinal cord tumor necrosis factor a and interleukin-1?content is less than ischemia-reperfusion group(P<0.05).5.MDA content and MPO activity ischemia and reperfusion in rat spinal cord tissue MDA,MPO activity was significantly higher than the sham group(P<0.05);by BML-111 treatment,MDA,MPO activity was significantly lower(P<0.05).Conclusions(1)By simultaneously opening the upper level of the left renal artery occlusion and the left and right common iliac artery bifurcation of the abdominal aorta to reach the lumbar spinal cord to block the main artery,SCII injury model can simulate the clinical maximum extent.(2)BML-111 on spinal cord ischemia-reperfusion injury model in rats has a protective effect and its mechanism may be related to inhibiting the expression and neutrophil infiltration of inflammatory cytokines,decrease neuronal apoptosis related. |
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