Effect Of Valproic Acid(VPA)on The Situ Activation Of Hippocampal Adut Neural Stem Cells(NSC)in Brain Tissue Of Rats With Experimental Traumatic Brain Injury

BackgroundTraumatic brain injury can cause different degree of brain injury,characterized by different neural function defect symptoms,even lead to death.Restoration of nerve function after traumatic brain injury,directly related to the patient’s prognosis and quality of life,which has became a key point in the research of neurosurgery.At present,the use of neural stem cells to repair traumatic brain injury provides a new direction in order to improve the nerve function defect,caused the high attention of research at home and abroad.Valproic acid is a line of clinical broad-spectrum antiepileptic drugs,in recent years,experimental studies have shown that valproic acid has significantly curative effect in the treatment of stroke,alzheimer’s disease,Parkinson’s disease and other diseases of the nervous system.This experiment by making severe head injury model in the rats,application of VPA to intervene,at different times determing the points of neurobehavioral scores,Edu in rats tissue cells and the expression of Nestin positive cells and its dynamic change of rats,observing the VPA on the activation function of neural stem cells in the adult hippocampus of cerebral injury in rats,to provide new experimental basis for nerve function repaired after brain injury applicating for VPA.ObjectivesThrough the determination of neurobehavioral scores,the number of Edu positive cells and the expression of Nestin positive cells in the hippocampal dentate gyrus of craniocerebral injury rats afte the valproic acid intervention.To observe the effect of valproic acid(VPA)on the nerve protective and hippocampal adut neural stem cells(NSC)in brain tissue of rats with experimental traumatic brain injury.MethodsSixty-six healthy male Sprague-Dawley(SD)rats were randomly divided into normal control group,the simple trauma group and VPA treated group.Rat model of closed head injury was established and then interfered by intraperitoneal injecting VPA 300mg·Kg-1 d-1.All the rats were determined neural function with reference to Neurological Severity Scores of rats and sacrificed by decapitation at 3,7,14,21,28 d after brain injury.Immunofluorescence was employed to detect the changes of 5-Ethynyl-2’-deoxyuridine(Edu)positive cells and Nestin positive cells in hippocampal dentate gyrus of each rats.Results1.Neurobehavioral scores:Neurobehavioral scores results showed that simple trauma group appeared different neural function defect symptoms in rats,when simple trauma group compared with normal control group at the same time,the difference was statistically significant(P < 0.05);the nerve function recovered of VPA group is better than the simple injury group in rats,and at the same time point comparing between the two groups,the differences was statistically significant(P < 0.05).2.Immunofluorescence:Immunofluorescence results showed that at the same time the Edu positive cells and the number of Nestin positive cellsthe in VPA treatment group were higher than that in normal control group and simple trauma group in the hippocampal dentate gyrus of rats,and the difference was statistically significant(P < 0.05).ConclusionsVPA has situ activation on hippocampal adut neural stem cells(NSC)in brain tissue of rats with experimental traumatic brain injury.