The Role Of XPD Gene In Proliferation, Migration And Expression Of PTEN Gene In SMMC-7721 Cells

Objective: To investigate the role of excision repair gene XPD(Xeroderma pigmentosum D) in proliferation, migration,apoptosisand and the expression of PTEN gene in human hepatocellular carcinoma SMMC-7721 cells after transfected with XPD.Methods: The empty plasmid pEGFP-N2 and recombinant plasmid pEGFPN2-XPD were transfected into human hepatoma cell line SMMC-7721 through Lipofectamine TM 2000 transiently.There are 4 groups:blank control group, liposome group, pEGFP-N2 group and pEGFP-N2-XPD group. The expression of green fluorescent protein gene was detected by fluorescence microscopy. Western blotting was used to measured the protein expression level of XPD and PTEN.MTT was used to observe the proliferation of tumor cells.Transwell assay were used to detect the migration capability of tumor cells. Flow cytometry was used to analyze the apoptosis of tumor cells.Results: The SMMC-7721 cells that transfected with recombinant plasmid pEGFP-N2-XPD and empty plasmid pEGFP-N2 emitted green fluorescence under the fluorescence microscope, indicating successful transfection. Western blotting,MTT,Transwell assay and flow cytometry demonstrated that compared with other three groups, the pEGFP-N2-XPD group showed significantly increased expression level of XPD and PTEN(p<0.01), decreased cell proliferation(p<0.01), decreased migration capability(p<0.05), and increased apoptosis rate(p<0.01).Conclusion: XPD can increase the expression of PTEN gene and inhibit proliferation, migration of hepatoma cells as well as induce apoptosis.