| Objective: To assess how well the seroconversion rates of antibodies for admitted kindergarten children after poliomyelitis(polio), whooping cough(pertussis), diphtheria and tetanus, hepatitis B virus(HBV) vaccine inoculations fit " timely vaccination rate, full vaccination rate, full and timely vaccination rate" via Enzyme-linked immunosorbent assay(ELISA). To evaluate the effects of vaccination and immunization for children. To provide a reliable basis for further improving immune planning strategy and measureing the quality of grassroots vaccination.Methods: Selecting 948 cases of newly admitted kindergarten children in Changleng Region of Xinjian County in 2015 as research objects, and detecting antibody positive level in their serum samples by indirect El ISA, and collecting their vaccination information through the identification of children vaccination certificate supplemented by questionnaire survey, and then proceeding the comparative analysis.1. On-site Survey: Child vaccination information including "first dose vaccination rate, vaccination rate of timely first dose, full-course vaccination rate, full-course timely vaccination rate" of Polio, Pertussis, Diphtheria and Tetanus, Hepatitis B Virus vaccines and other information. Specifically:(1) Locally census-registered children within the jurisdiction: To collect planning information by Jiangxi Province Immune System including the child’s name, sex, date of birth, parents’ names and other relevant information to verify the vaccination information of admission children, and auxiliary on-site questionnaires and informed consent forms for confirming vaccination, household register, place of residence and other information of admission children, and establishing a strictly logic verification method.(2) Census-registered children outside the jurisdiction: EPI(Expanded Programme on Immunization) information could not be tracked due to national network not established, so to gather the above information through informing and investigating parents of the kindergarteners with vaccination cards, if individuals have to work and so can not go to the kindergarten we intends to ring up or visit them to resolve.2. Indirect ELISA: Microplates coated with purified Poliovirus Ⅰ,Ⅱ,Ⅲ antigens, Pertussis Filamentous Hemagglutinin(FHA) and Pertussis toxin(PT) component subunit antigens, Diphtheria toxoid, Tetanus toxoid toxin, Hepatitis B Virus antigens, and the corresponding enzyme-marked human IgG monoclonal antibody with the principle of indirect ELISA to test the concentration of anti-poliovirus Ⅰ,Ⅱ,Ⅲ type IgG antibodies, pertussis IgG antibodies, diphtheria toxoid IgG antibody, tetanus toxoid IgG antibody, hepatitis B virus IgG antibodies in the sample serum.3. Statistical analysis: Using SPSS19.0 software for statistical analysis. Wherein the count data used chi-square test, P<0.05 shows that the difference was significant; P> 0.05 shows no significant difference.Results:1. The seroconversion rates of antibodies of hepatitis B, polio, pertussis, diphtheria and tetanus vaccine among 948 cases of admission children were 64.14%, 99.79%, 99.58%, 93.14% and 93.99%, respectively.2. Among 948 cases of admission children, 589 male children seroconversion rates of antibodies of hepatitis B, polio, pertussis, diphtheria and tetanus vaccine were in turns 62.82%, 100%, 99.49%, 93.04% and 93.72%; 359 female children were: 66.30%, 99.44%, 99.72%, 93.01% and 94.43% accordingly. Gender seroconversion was no significant difference(P> 0.05).3. Among 836 cases of admission children, the 699 census-registered children of Xinjian District vaccinated with hepatitis B, polio, pertussis, diphtheria and tetanus vaccine obtained 64.95%, 99.86%, 99.71%, 93.13% and 93.71% of seroconversion rates respectively; the 137 non census-registered children of Xinjian District did 62.04%, 99.27,98.54%, 91.97% and 95.62% accordingly. The seroconversion rates for the census register children of Xinjian District and the census register children outside Xinjian District were no significant difference(P> 0.05).4. Among 831 cases of admission children, the seroconversion rates of rural children vaccinated with hepatitis B, polio, pertussis, diphtheria and tetanus vaccine were 59.72, 99.65%, 99.31%, 90.28% and 91.32 in turns. Urban children were 67.03%, 99.82%, 99.63%, 94.84% and 95.58% accordingly. Urban children diphtheria had significantly higher seroconversion rate than rural children(χ2 value = 6.288; P<0.05). Tetanus antibody positive rate of urban children was significantly higher than that of rural children(χ2 value=6.206; P<0.05). Rural children and urban children with hepatitis B, polio, pertussis seroconversion rate were no significant difference(P> 0.05).5. The seroconversion rates of 948 cases of admission children vaccinated with the first dose of hepatitis B, polio, pertussis, diphtheria and tetanus vaccine timely were 64.23%, 99.78%、99.35%、93.20% and 94.50% respectively; the children who were not vaccinated in time had 63.93%, 99.80%, 99.69%, 93.11% and 93.75% accordingly. Timely and not timely vaccination with the first dose had no significant difference of seroconversion rate(P>0.05).6. The vaccination seroconversion rates of the whole 948 cases of admission children vaccinated with hepatitis B, polio, pertussis, diphtheria and tetanus, were 64.83%, 99.78%, 99.64%, 94.87% and 95.95% respectively; accordingly, the seroconversion rates of those who did not have a complete course of vaccination were 48.78%, 100.00%, 99.08%, 79.82% and 78.90%. Hepatitis B antibody positive rates for children who had a complete course of vaccination and those who did not do were significantly different(χ2 value = 4.392; P<0.05). Diphtheria antibody seroconversion rates for the above two group children were significantly different(χ2 value = 34.251; P <0.01). Antibody seroconversion rates of tetanus vaccination for the above two group children were significantly different(χ2 value= 49.615; P<0.01). However, polio, pertussis seroconversion rates for the above two group children were no significant difference(P> 0.05).7. Among 948 cases of children vaccinated hepatitis B, polio, pertussis, diphtheria and tetanus after vaccination, the seroconversion rates for timely vaccination were 64.50%, 100.00%, 100.00%, 93.98% and 96.24%; the seroconversion rates for delayed vaccination were respectively 64.06%, 99.74%, 99.51%, 93.01% and 93.62%. Timely vaccination compare to delayed vaccination had no significant difference of seroconversion rate(P> 0.05).Conclusion:1. In the survey of the 948 cases of admitted kindergarten children in Changleng Region of Xinjian County in 2015, the seroconversion rates of polio, pertussis, diphtheria and tetanus vaccine were all over 90%, which shows a satisfactory immune effect. But hepatitis B antibody positive seroconversion rate was a relatively lower, indicating that this region should continue to strengthen the detection and inoculation of hepatitis B vaccine.2. In the survey, in addition to that urban children had significantly higher antibody positive rates than rural ones after inoculated against diphtheria and tetanus vaccine, gender, household register, place of residence have little impact on antibody seroconversion rates of hepatitis B, polio, whooping cough, diphtheria and tetanus vaccine.3. In the survey, compare to children who did not have a complete course of vaccination, those whose had a complete course obviously achieved much higher seroconversion rates of antibodies of hepatitis B, diphtheria and tetanus vaccines4. In the survey, first dose timely vaccination, the entire process timely vaccination of admission children had little impact on the seroconversion rates of IgG antibodies against hepatitis B,polio,pertussis,diphtheria and tetanus.The results of above investigation are expected to provide a reference for the Xinjian County(district) Evaluation of admission child immunization, and to provide theoretical and experimental basis for further improving child immunization program. |
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