| In recent years, cardiovascular diseases have seriously threatened people’s health and lives. In order to ensure the regular vessel blood supply, vascular grafts have been used to solve this problem. Due to the clinical limitation of autologous vascular transplantation and allograft vascular transplantation, fabricating the artificial vascular grafts with excellent biocompatibility and mechanical properties has become a current research hotspot.Electrospinning technology can prepare nanofibers with high surface area and porosity to mimic the 3-D structure of extracellular matrix and has become one efficient method to construct artificial scaffolds. In this dissertation, electrospinning technology was employed to prepare tissue engineering blood scaffold using poly(lactic-co-glycolic acid)(PLGA) and silk fibroin(SF) as raw materials and hexafluoroisopropanol(HFIP) as spinning solvent. Electrospraying technology was also used to modify the surface of fibrous scaffolds to promote the rapid endothelialization of scaffolds. First, we fabricated PLGA/SF fibrous scaffolds with various weight ratios, then verifying the most proper weight ratio through testing the physical and chemical properties of different PLGA/SF scaffolds. In order to promote endothelialization and inhibit thrombosis and restenosis, the microparticles(MPs/pZNF580) were used to modify the surface of PLGA/SF scaffolds by electrospraying technology. Furthermore, the influence of these microparticles on the bio-performance of the scaffolds was explored.The molecular composition, surface appearance, diameters and distribution, hydrophilicity, tensile strength and elongation at break were characterized by FTIR, SEM, water contact angle, water absorption and mechanical properties, respectively. Thus, the optimal ratio of PLGA/SF was found and used for further modification. SEM was utilized to observe the surface of fibrous scaffolds before and after modification. The proliferation of cells on the composite scaffolds was studied by the incubation of HUVECs and HUASMCs culture.The results indicated that PLGA/SF scaffold with weight ratio of 70/30, with fiber diameter of 538.1 ± 147.5 nm, water contact angle of 55.0 ± 2.3°, water absorption of 84.4 ± 6.7%, tensile strength of 1.5 ± 0.1 MPa and elongation at break of 77.4 ± 6.4%, was optimal for tissue engineering scaffolds. Cell culture experiment indicated that the scaffolds modified with MPs/pZNF580 had almost non-cytotoxicity. The modified scaffolds could enhance the proliferation of HUVECs, and inhibit the proliferation of HUASMCs. These results showed that the composite scaffolds had a potential application in tissue engineering. |
