Radiosensitization And Its Mechanism Of Down Regulation Musashi1 In Colon Carcinoma Cell Line HCT116

Objective: The technology of RNA interference was used to knockdown the expression of Musashi1 in HCT116 cell line,and then exposed to radiation,so that to discuss whether down regulation Musashi1 can sensitizes human colon carcinoma cell line HCT116 to radiation,which can provide an evidence for the clinical treatment.Method:1 Lentiviral vector was used to knockdown the expression of Musashi1 in HCT116 cell line?HCT116-Musashi1?,and establish the negative group.2 Colony formation assay was used to detect the radiosensitization of down regulation Musashi1 in colon carcinoma cell line HCT116.3 MTS assay was used to detect the cell proliferation activity of Silence group,Control group and NC group after accepting 8Gy irradiation.4 Flow cytometry was used to measure the cell cycle distribution after exposing to 12 Gy irradiated at 48 h,and apoptosis rate changes among Silence group,Control group and NC group after accepting 8Gy irradiation at 24 h,48h and 72 h.Result:1 Colony formation assays result showed that the radiation sensitivity of Silence group was significantly higher than Control and NC,however there was no difference of the two others.2 MTS assays showed that the cell proliferation of three groups decreased after 8Gy irradiation at 24 h 48h 72 h,however Silence group's growth rate was always slower than Control and NC?P<0.001?,there was no difference between Control and NC?P>0.05?.And the inhibition ratio of cell proliferation among three groups were all time depended,but the inhibition ratio of cell proliferation of Silence group was always higher than others?P<0.05?.3 After 8Gy irradiation,the apoptosis rates increased,which Silence group's apoptosis rate increased faster than other two groups at 24 h 48h 72h?P<0.001?,and with time increasing,the apoptosis rates increased?P<0.001?,which Silence group's apoptosis rate always increased faster than other two groups.4 Cell cycle experiment showed that after 12 Gy irradiation at 48h,G₂/M phase decreased significantly in Silence group compared with Control group and NC group?P<0.001?.Conclusion:1 Knockdown the expression of Musashi1 in HCT116 cell can increase the sensitivity of radiotherapy;2 Knockdown the expression of Musashi1 in HCT116 cell can increase the sensitivity of radiation,through promoting cell apoptosis and reversing G₂/M arrest.