The Proliferation Study Of IncRNA FOXCUT Regulation To FOXC1 In Breast Cancer

BackgroundBreast cancer is the most common malignant tumor of women. The prevalence rate of breast cancer is on rise year by year [33]. Pre-cancerous lesions of breast cancer patients have a good effect after surgical removal, radiation and endocrine therapy,but these methods can do only so much when tumor cells hidein plain sight and even a single overlooked cell can seed new disease.Therefore,almost all the patients must to face the relapse concerns and metastasis,which cause poor prognosis. Recently a lot of research confirmed that FOXC1 expressed in many cancer tissues, such as hepatocellular carcinoma, breast cancer, gastric cancer, non-small cell lung cancer, and the high expression of FOXC1 is closely related to the poor prognosis of these cancers. IncRNA is a highly conserved transcript of messenger-like rna,it can not encode protein, exist in the cell nucleus, and has no open reading frames, and exist in the cell nucleus or cytoplasm. There are a variety of connections between IncRNA and neighboring mRNA both in expression and function.They are exsit in the form of a IncRNA-mRNA pair, to play the role of interaction and function.ObjectiveTo analyse the expression of FOXC1 and IncRNA FOXCUT in breast carcinoma tissue and normal breast tissue, To explore the correlation between FOXC1 and its adjacent long non coding RNA(long noncode RNA, IncRNA).To observe the relationship of FOXCUT and FOXC1 with clinical pathological factors and prognosis of breast cancer patients, providing a new theoretical basis for treatment and prognosis of breast cancer.Methods1. Using Western blot and ICH detceted the expression of FOXC 1 in 65 specimen of breast cancer tissues.2. Using Real time PCR to detect FOXC1 and FOXCUT expression levels in breast cancer tissues and corresponding adjacent normal tissues; as well as to study the correlation between FOXC1 and FOXCUT.3. To establish siRNAs (small interfering RNA, siRNA) by knocking down the expression of FOXC1 and FOXCUT. Realtime-PCR and Western blot were used to detect the expression and correlation of FOXC1 and FOXCUT.4. Using the method of CCK-8 tested MCF-7 cells vitality, after establishing siRNA by knocking down the expression of FOXC1 and FOXCUT.Results1. The expression of FOXC1 and FOXCUT in breast cancer tissues was higher than that the adjacent normal tissues.2. The expression of FOXC1 and FOXCUT in breast cancer tissue has a correlation between the two genes in the form of gene function, down regulated FOXC1 can inhibit the expression of FOXCUT.3. The downregulation of FOXC1 and FOXCUT expression can inhibit the proliferation of MCF-7 cells.Conclusion1. There is exsisting interaction between FOXC1 and FOXCUT in breast cancer.2. FOXC1 and FOXCUT can regulate the proliferation of breast cancer cells by FOXCUT-FOXC1 gene.3. FOXC1 and FOXCUT can be used as a marker for breast cancer and a new target for gene therapy.