Correlation Of Msi-1 In Colorectal Cancer Cells And Pathological Characteristics Of Colorectal Cancer

Objective: Expression studies in colorectal cancer Msi-1 gene, combined with the pathological features of patients gathered to further clarify the relationship Msi-1 gene in colorectal cancer growth. Study the effect of silencing Msi-1 gene expression of the biological characteristics of human colorectal carcinoma LOVO cell cycle, proliferation, apoptosis,invasion and metastasis and its mechanism.Methods: Cultured colon cancer cells HT-29, DLD-1, LOVO, screening cells expressing moderate; synthetic interfering RNA, LOVO transfected cells, transfection efficiency was observed by flow cytometry, PCR and Msi-1 mRNA sequence designed for Msi-1 mRNA expression and protein gene detected interference Western Blot method. CCK-8 cell proliferation, Transwell migration and invasion of tumor cell testing, analysis silence tumor proliferation, migration and invasion of differences; Flow measuring machine cycle and apoptosis of cells was Msi-1 gene silencing after. Western blot detection of OPN protein,CD44, and with the co-expression of Msi-1 situation.Results: Immunohistochemistry showed that colorectal cancer tissues of Msi-1expression were increased larger than normal mucosa Msi-1 expression levels of many.LOVO cell expression of Msi-1 gene is moderate, silencing efficiency interfering RNA sequences 621 set up, transfection interference sequence, the expression of Msi-1 gene in RNA and protein levels were significantly decreased in colorectal cancer LOVO interference after, cell proliferation, migration and invasion were significantly decreased, colorectal cancer and OPN and CD44 in RNA and protein expression levels were down.Conclusion: After the use of siRNA transfected cells, can be found in the expression of LOVO cells Msi-1 gene was significantly inhibited. Msi-1 gene silencing after being suppressed LOVO human colorectal cancer cell growth and promote apoptosis in colon cancer cells, the cell cycle increase in G0 / G1 phase of the cell, with a deterrent effect, in metastatic colorectal cancer also plays a effect.