Expression Of 4.1R Protein MRNA In Myocardial Tissues Of Elderly Mice With Heart Failure

ObjectiveHeart failure is due to cardiac dysfunction or structural abnormal lead to systolic or diastolic dysfunction is the variety of end stage heart disease.With the aging of our population has been increasing degree of heart failure morbidity and mortality also increased significantly. Protein 4.1 is an important component of red cell membrane skeleton, it plays an important role in maintaining red blood cell morphology. Protein 4.1 can affect the localization of ion channels, which regulate the connection between cells through the cell adhesion function. Recent studies have shown that damage to the contractile protein-based membrane skeleton structure may lead to cardiac dysfunction, therefore, protein 4.1 attracted the attention of more and more researchers. In this study, we use RT-PCR technology to detect expression of 4.1R protein m RNA in myocardial tissue, exploring the relationship between 4.1R and heart failure. MethodsWe divided a total of 40 healthy elderly male mice into heart failure group(n = 20) and control group(n = 20). HF group use abdominal aortic coarctation to established mouse model of heart failure.The control group did not undergo abdominal aorta after laparotomy. After surgery were routinely fed 8 weeks.After 8 weeks,we removed their hearts to assess the left ventricular mass index(LVMI) after we executed them. We used RT-PCR to detect the expression of 4.1R protein in elderly mice myocardial tissue to compare the differences between the two group. The statistical data was analyzed by t-test or rank sum test.Value P<0.01 was considered to be significant. Results1.General comparison: HF groups were occurred varying degrees of shortness of breath, wheezing intermittently, reduced activity levels after eight weeks.2.The comparison of left ventricular miss index(LVMI):in the HF group,the index was 5.72±0.42,in the control group,the index was 3.07±0.29,comparing the control group,the LVMI of the HF group increased(P<0.01).3.Hematoxylin and eosin stain:in the control group,myocardial cells were structural integrity, tightly packed cells, muscle fibers arranged in neat rows.In the HF group,myocardial cells showed varying degrees of swelling and hypertrophy, myocardial fibers disarray, breaking limitations, cell gap widened, part of the heart or was focal necrosis.4.Ultrasonography:Compared with the control group, the heart rate, LVESD, LVEDD, left ventricular mass index in heart failure group markedly increased(P<0.01).5.RT-PCR:the expression of 4.1R protein m RNA significantly increased in myocardial tissue of the heart failure group, and the differences were statistically significant. Conclusion1.Abdominal aortic coarctation can be successfully established mouse model of heart failure.2.The significantly increased expression of 4.1R protein m RNA in myocardial tissue of elderly mice with HF suggests that 4.1R protein may be related to the occurrence and development of heart failure and farther studies are needed.